Human Vaccinia Related Kinase 1 (VRK1) ELISA Kit | VRK1 elisa kit

Human Vaccinia Related Kinase 1 (VRK1) ELISA Kit

Cat. Num. AAA2704557

• Gene Names: VRK1; PCH1; PCH1A
• Reactivity: Human
• Synonyms: Vaccinia Related Kinase 1 (VRK1); Human Vaccinia Related Kinase 1 (VRK1) ELISA Kit; Serine/threonine-protein kinase VRK1; VRK1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vaccinia Related Kinase 1 (VRK1).; No significant cross-reactivity or interference between Vaccinia Related Kinase 1 (VRK1) and analogues was observed.
• Sequence Length: 396

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vaccinia Related Kinase 1 (VRK1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vaccinia Related Kinase 1 (VRK1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VRK1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vaccinia Related Kinase 1 (VRK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vaccinia Related Kinase 1 (VRK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vaccinia Related Kinase 1 (VRK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vaccinia Related Kinase 1 (VRK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 109730507
• NCBI GeneID: 7443
• UniProt Accession #: Q99986; Q3SYL2
• Molecular Weight: 45,476 Da
• NCBI Official Full Name: Vaccinia related kinase 1
• NCBI Official Synonym Full Names: vaccinia related kinase 1
• NCBI Official Symbol: VRK1
• NCBI Official Synonym Symbols: PCH1; PCH1A
• NCBI Protein Information: serine/threonine-protein kinase VRK1
• UniProt Protein Name: Serine/threonine-protein kinase VRK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: VRK1

NCBI Description

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.