Human Endothelial Cell Specific Molecule 1 (ESM1) ELISA Kit | ESM1 elisa kit

Human Endothelial Cell Specific Molecule 1 (ESM1) ELISA Kit

Cat. Num. AAA2704465

• Gene Names: Esm1; ESM-1; AV004503; 0610042H23Rik
• Reactivity: Human
• Synonyms: Endothelial Cell Specific Molecule 1 (ESM1); Human Endothelial Cell Specific Molecule 1 (ESM1) ELISA Kit; Endocan; ESM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ESM1. No significant cross-reactivity or interference between ESM1 and analogues was observed.
• Sequence Length: 184

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ESM1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ESM1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ESM1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ESM1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ESM1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ESM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ESM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ESM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14196342
• NCBI GeneID: 71690
• NCBI Accession #: NP_076101.1
• NCBI GenBank Nucleotide #: NM_023612.3
• UniProt Accession #: Q9QYY7
• Molecular Weight: 20,043 Da
• NCBI Official Full Name: endothelial cell-specific molecule 1
• NCBI Official Synonym Full Names: endothelial cell-specific molecule 1
• NCBI Official Symbol: Esm1
• NCBI Official Synonym Symbols: ESM-1; AV004503; 0610042H23Rik
• NCBI Protein Information: endothelial cell-specific molecule 1
• UniProt Protein Name: Endothelial cell-specific molecule 1
• Protein Family: Endothelial cell-specific molecule
• UniProt Gene Name: Esm1
• UniProt Synonym Gene Names: ESM-1

Uniprot Description

Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions ().