Human Growth Differentiation Factor 15 (GDF15) ELISA Kit | GDF15 elisa kit

Human Growth Differentiation Factor 15 (GDF15) ELISA Kit

Cat. Num. AAA2704091

• Gene Names: GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
• Reactivity: Human
• Synonyms: Growth Differentiation Factor 15 (GDF15); Human Growth Differentiation Factor 15 (GDF15) ELISA Kit; PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor; GDF15 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Differentiation Factor 15 (GDF15).; No significant cross-reactivity or interference between Growth Differentiation Factor 15 (GDF15) and analogues was observed.
• Sequence Length: 308

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 15 (GDF15) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 15 (GDF15) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF15 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 15 (GDF15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 15 (GDF15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Differentiation Factor 15 (GDF15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Differentiation Factor 15 (GDF15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14286322
• NCBI GeneID: 9518
• UniProt Accession #: Q99988; O14629; P78360; Q9BWA0; Q9NRT0
• Molecular Weight: 34,140 Da
• NCBI Official Full Name: Growth differentiation factor 15
• NCBI Official Synonym Full Names: growth differentiation factor 15
• NCBI Official Symbol: GDF15
• NCBI Official Synonym Symbols: PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
• NCBI Protein Information: growth/differentiation factor 15
• UniProt Protein Name: Growth/differentiation factor 15
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF15
• UniProt Synonym Gene Names: MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress. [provided by RefSeq, Aug 2016]