Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA Kit | ACP5 elisa kit

Rat Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA Kit

Cat. Num. AAA2702692

• Gene Names: ACP5; HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
• Reactivity: Rat
• Synonyms: Acid Phosphatase 5; Tartrate Resistant (ACP5); Rat Acid Phosphatase 5; Tartrate Resistant (ACP5) ELISA Kit; TRAP; TrATPase; Tartrate Resistant Acid Phosphatase; Type 5 acid phosphatase; ACP5 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ACP5. No significant cross-reactivity or interference between ACP5 and analogues was observed.
• Sequence Length: 325

• Samples: Mouse Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.39-25ng/mL
• Sensitivity: 0.15ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ACP5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ACP5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACP5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACP5 in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ACP5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ACP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ACP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ACP5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 83405786
• NCBI GeneID: 54
• Molecular Weight: 36,599 Da
• NCBI Official Full Name: Acid phosphatase 5, tartrate resistant
• NCBI Official Synonym Full Names: acid phosphatase 5, tartrate resistant
• NCBI Official Symbol: ACP5
• NCBI Official Synonym Symbols: HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
• NCBI Protein Information: tartrate-resistant acid phosphatase type 5
• UniProt Protein Name: Tartrate-resistant acid phosphatase type 5
• Protein Family: Acyl carrier protein
• UniProt Gene Name: ACP5
• UniProt Synonym Gene Names: TR-AP; TrATPase

NCBI Description

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

Uniprot Description

Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.