Human Keratin 16 (KRT16) ELISA Kit | KRT16 elisa kit

Human Keratin 16 (KRT16) ELISA Kit

Cat. Num. AAA2701884

• Gene Names: KRT16; K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• Reactivity: Human
• Synonyms: Keratin 16 (KRT16); Human Keratin 16 (KRT16) ELISA Kit; CK16; NEPPK; Cytokeratin 16; Focal Non-Epidermolytic Palmoplantar Keratoderma; Keratin; type I cytoskeletal 16; KRT16 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 16 (KRT16).; No significant cross-reactivity or interference between Keratin 16 (KRT16) and analogues was observed.
• Sequence Length: 473

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.064ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 16 (KRT16) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 16 (KRT16) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT16 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 16 (KRT16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 16 (KRT16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 16 (KRT16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 16 (KRT16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 24430192
• NCBI GeneID: 3868
• NCBI Accession #: NP_005548.2
• NCBI GenBank Nucleotide #: NM_005557.3
• UniProt Accession #: P08779; P30654; Q16402; Q9UBG8; A8K488
• Molecular Weight: 51,268 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 16
• NCBI Official Synonym Full Names: keratin 16
• NCBI Official Symbol: KRT16
• NCBI Official Synonym Symbols: K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• NCBI Protein Information: keratin, type I cytoskeletal 16
• UniProt Protein Name: Keratin, type I cytoskeletal 16
• Protein Family: Keratin
• UniProt Gene Name: KRT16
• UniProt Synonym Gene Names: KRT16A; CK-16; K16

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]

Uniprot Description

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.