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Typical Testing Data/Standard Curve (for reference only)

Rat Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit | aHSG elisa kit

Rat Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit

Gene Names
AHSG; AHS; A2HS; HSGA; FETUA
Reactivity
Rat
Synonyms
Alpha-2-Heremans Schmid Glycoprotein (aHSG); Rat Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit; HSGa; A2HS; AHS; FETUA; HSG-A2; Fetuin-A; Alpha 2-HS Glycoprotein; Fetuin A; Alpha-2-Z-Globulin; Ba-alpha-2-Glycoprotein; aHSG elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Alpha-2-Heremans Schmid Glycoprotein (aHSG).
No significant cross-reactivity or interference between Alpha-2-Heremans Schmid Glycoprotein (aHSG) and analogues was observed.
Sequence Length
367
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
Detection Range
6.25-400ng/mL
Sensitivity
< 2.55ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Alpha-2-Heremans Schmid Glycoprotein (aHSG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Alpha-2-Heremans Schmid Glycoprotein (aHSG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for aHSG elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Heremans Schmid Glycoprotein (aHSG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Heremans Schmid Glycoprotein (aHSG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Heremans Schmid Glycoprotein (aHSG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Heremans Schmid Glycoprotein (aHSG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
197
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,325 Da
NCBI Official Full Name
alpha-2-HS-glycoprotein isoform 2 preproprotein
NCBI Official Synonym Full Names
alpha 2-HS glycoprotein
NCBI Official Symbol
AHSG
NCBI Official Synonym Symbols
AHS; A2HS; HSGA; FETUA
NCBI Protein Information
alpha-2-HS-glycoprotein
UniProt Protein Name
Alpha-2-HS-glycoprotein
Protein Family
UniProt Gene Name
AHSG
UniProt Synonym Gene Names
FETUA

NCBI Description

The protein encoded by this gene is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including endocytosis, brain development, and the formation of bone tissue. Defects in this gene are a cause of susceptibility to leanness. [provided by RefSeq, Aug 2017]

Uniprot Description

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27.3

Cellular Component: blood microparticle; endoplasmic reticulum lumen; extracellular exosome; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; secretory granule lumen

Molecular Function: cysteine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; kinase inhibitor activity

Biological Process: acute-phase response; cellular protein metabolic process; negative regulation of bone mineralization; negative regulation of endopeptidase activity; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; neutrophil degranulation; ossification; pinocytosis; platelet degranulation; positive regulation of phagocytosis; post-translational protein modification; regulation of bone mineralization; regulation of inflammatory response; skeletal system development

Research Articles on aHSG

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Product Notes

The Rat aHSG ahsg (Catalog #AAA2701452) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2701452 ELISA Kit recognizes Rat aHSG. It is sometimes possible for the material contained within the vial of "Alpha-2-Heremans Schmid Glycoprotein (aHSG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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