Bovine Pepsinogen A (PGA) ELISA Kit | PGA elisa kit

Cattle Pepsinogen A (PGA) ELISA Kit

Cat. Num. AAA2701420

• Gene Names: PGA5; Pg5
• Reactivity: Bovine
• Synonyms: Pepsinogen A (PGA); Cattle Pepsinogen A (PGA) ELISA Kit; PGA5; PG-A; Pepsinogen 5 Group I; Pepsinogen-5; Pepsin A-5; PGA elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pepsinogen A (PGA).; No significant cross-reactivity or interference between Pepsinogen A (PGA) and analogues was observed.
• Sequence Length: 388

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.108ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pepsinogen A (PGA) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pepsinogen A (PGA) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsinogen A (PGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pepsinogen A (PGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pepsinogen A (PGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pepsinogen A (PGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 387013
• NCBI GeneID: 5222
• UniProt Accession #: P0DJD9; P00790; Q7M4R0; Q8N1E3; A8K749; B7ZW62; B7ZW75
• Molecular Weight: 41,993 Da
• NCBI Official Full Name: pepsinogen A
• NCBI Official Synonym Full Names: pepsinogen 5, group I (pepsinogen A)
• NCBI Official Symbol: PGA5
• NCBI Official Synonym Symbols: Pg5
• NCBI Protein Information: pepsin A-5
• UniProt Protein Name: Pepsin A-5
• Protein Family: Pepsin
• UniProt Gene Name: PGA5

NCBI Description

This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]

Uniprot Description

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.