Mouse Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit | SOD3 elisa kit

Mouse Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit

Cat. Num. AAA2701263

• Gene Names: SOD3; EC-SOD
• Reactivity: Mouse
• Synonyms: Superoxide Dismutase 3; Extracellular (SOD3); Mouse Superoxide Dismutase 3; Extracellular (SOD3) ELISA Kit; SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn; SOD3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 3, Extracellular (SOD3).; No significant cross-reactivity or interference between Superoxide Dismutase 3, Extracellular (SOD3) and analogues was observed.
• Sequence Length: 300

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOD3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 3, Extracellular (SOD3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase 3, Extracellular (SOD3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119613229
• NCBI GeneID: 6649
• UniProt Accession #: P08294; Q5U781; Q6FHA2
• Molecular Weight: 25,851 Da
• NCBI Official Full Name: superoxide dismutase 3, extracellular
• NCBI Official Synonym Full Names: superoxide dismutase 3
• NCBI Official Symbol: SOD3
• NCBI Official Synonym Symbols: EC-SOD
• NCBI Protein Information: extracellular superoxide dismutase [Cu-Zn]
• UniProt Protein Name: Extracellular superoxide dismutase [Cu-Zn]
• Protein Family: Extracellular superoxide dismutase
• UniProt Gene Name: SOD3
• UniProt Synonym Gene Names: EC-SOD

NCBI Description

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the conversion of superoxide radicals into hydrogen peroxide and oxygen, which may protect the brain, lungs, and other tissues from oxidative stress. Proteolytic processing of the encoded protein results in the formation of two distinct homotetramers that differ in their ability to interact with the extracellular matrix (ECM). Homotetramers consisting of the intact protein, or type C subunit, exhibit high affinity for heparin and are anchored to the ECM. Homotetramers consisting of a proteolytically cleaved form of the protein, or type A subunit, exhibit low affinity for heparin and do not interact with the ECM. A mutation in this gene may be associated with increased heart disease risk. [provided by RefSeq, Oct 2015]

Uniprot Description

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.