Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Chicken pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit | PACAP elisa kit

Chicken pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit

Gene Names
ADCYAP1; PACAP
Reactivity
Chicken
Synonyms
pituitary adenylate cyclase activating polypeptide (PACAP); Chicken pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit; PACAP elisa kit
Ordering
For Research Use Only!
Reactivity
Chicken
Specificity
No cross-reaction with other factors.
Sequence Length
176
Samples
Chicken Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Opg Concentration
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
0.05 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PACAP elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken OPG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
116
Molecular Weight
18,835 Da
NCBI Official Full Name
pituitary adenylate cyclase activating polypeptide
NCBI Official Synonym Full Names
adenylate cyclase activating polypeptide 1 (pituitary)
NCBI Official Symbol
ADCYAP1
NCBI Official Synonym Symbols
PACAP
NCBI Protein Information
pituitary adenylate cyclase-activating polypeptide; prepro-PACAP
UniProt Protein Name
Pituitary adenylate cyclase-activating polypeptide
UniProt Gene Name
ADCYAP1
UniProt Synonym Gene Names
PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38
UniProt Entry Name
PACA_HUMAN

NCBI Description

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

Uniprot Description

ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 18p11

Cellular Component: extracellular space; extracellular region; terminal button

Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor signaling protein activity; pituitary adenylate cyclase-activating peptide receptor binding; pituitary adenylate cyclase activating polypeptide activity; receptor binding

Biological Process: behavioral fear response; nerve growth factor receptor signaling pathway; negative regulation of potassium ion transport; female pregnancy; sensory perception of pain; positive regulation of vasodilation; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell cycle; histamine secretion; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; regulation of protein localization; cell-cell signaling; ovarian follicle development; neuropeptide signaling pathway; positive regulation of cell proliferation; neurite development; negative regulation of acute inflammatory response to antigenic stimulus; adenylate cyclase activation; positive regulation of interleukin-6 production; positive regulation of growth hormone secretion; ATP metabolic process; response to starvation; response to ethanol; regulation of postsynaptic membrane potential; pituitary gland development; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of acute inflammatory response to non-antigenic stimulus; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of G-protein coupled receptor protein signaling pathway

Research Articles on PACAP

Similar Products

Product Notes

The Chicken PACAP adcyap1 (Catalog #AAA269153) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA269153 ELISA Kit recognizes Chicken PACAP. It is sometimes possible for the material contained within the vial of "pituitary adenylate cyclase activating polypeptide (PACAP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.