Human Myelin Basic Protein Antibody (MBP Ab) ELISA Kit | MBP Ab elisa kit

Human Myelin Basic Protein Antibody (MBP Ab) ELISA Kit

Cat. Num. AAA267884

• Gene Names: MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• Reactivity: Human
• Synonyms: Myelin Basic Protein Antibody (MBP Ab); Human Myelin Basic Protein Antibody (MBP Ab) ELISA Kit; MBP Ab elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 248

• Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant MBP Ab concentration.
• Assay Type: Sandwich
• Detection Range: 100 U/ml-1.56 U/ml
• Sensitivity: 0.5 U/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for MBP Ab elisa kit

Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MBP Ab monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557739
• NCBI GeneID: 4153
• NCBI Accession #: NP_000233.1
• NCBI GenBank Nucleotide #: NM_000242.2
• Molecular Weight: 26,144 Da
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose-binding lectin (protein C) 2, soluble
• NCBI Official Symbol: MBL2
• NCBI Official Synonym Symbols: MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• NCBI Protein Information: mannose-binding protein C; collectin-1; mannan-binding lectin; mannose-binding lectin 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble (opsonic defect)
• UniProt Protein Name: Mannose-binding protein C
• UniProt Gene Name: MBL2
• UniProt Synonym Gene Names: COLEC1; MBL; MBP-C
• UniProt Entry Name: MBL2_HUMAN

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; cell surface; collagen; extracellular region

Molecular Function: mannose binding; protein binding; calcium ion binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; positive regulation of phagocytosis; defense response to bacterium; acute-phase response; innate immune response; response to oxidative stress; opsonization; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction; complement activation

Disease: Mannose-binding Protein Deficiency