Mouse Glutathione (GSH) ELISA Kit | GSH elisa kit

Mouse Glutathione (GSH) ELISA Kit

Cat. Num. AAA267424

• Gene Names: GSTM2; GST4; GSTM; GTHMUS; GSTM2-2
• Reactivity: Mouse
• Synonyms: Glutathione (GSH); Mouse Glutathione (GSH) ELISA Kit; GSH elisa kit

• Reactivity: Mouse
• Sequence Length: 191

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 100 mmol/L-1.56 mmol/L
• Sensitivity: Up to 0.5 mmol/L
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSH elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse GSH monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 215277000
• NCBI GeneID: 2946
• NCBI Accession #: NP_001135840.1
• NCBI GenBank Nucleotide #: NM_001142368.1
• Molecular Weight: 22,644 Da
• NCBI Official Full Name: glutathione S-transferase Mu 2 isoform 2
• NCBI Official Synonym Full Names: glutathione S-transferase mu 2 (muscle)
• NCBI Official Symbol: GSTM2
• NCBI Official Synonym Symbols: GST4; GSTM; GTHMUS; GSTM2-2
• NCBI Protein Information: glutathione S-transferase Mu 2; GST, muscle; GST class-mu 2; glutathione S-transferase 4; glutathione S-transferase M1; glutathione S-aryltransferase M2; glutathione S-alkyltransferase M2; glutathione S-aralkyltransferase M2; S-(hydroxyalkyl)glutathione lyase M2; glutathione S-transferase M2 (muscle)
• UniProt Protein Name: Glutathione S-transferase Mu 2
• UniProt Gene Name: GSTM2
• UniProt Synonym Gene Names: GST4
• UniProt Entry Name: GSTM2_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family.

Protein type: Transferase; EC 2.5.1.18; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: sarcoplasmic reticulum; cytoplasm; cytosol

Molecular Function: enzyme binding; protein homodimerization activity; glutathione binding; glutathione transferase activity; receptor binding

Biological Process: regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; glutathione metabolic process; nitrobenzene metabolic process; xenobiotic metabolic process; xenobiotic catabolic process