Mouse Aquaporin 3 (AQP-3) ELISA Kit | AQP-3 elisa kit

Mouse Aquaporin 3 (AQP-3) ELISA Kit

Cat. Num. AAA267212

• Gene Names: AQP3; GIL; AQP-3
• Reactivity: Mouse
• Synonyms: Aquaporin 3 (AQP-3); Mouse Aquaporin 3 (AQP-3) ELISA Kit; AQP-3 elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 3

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AQP-3 concentration
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: 0.05ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-3 elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse AQP-3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4433117
• NCBI GeneID: 360
• Molecular Weight: 29,987 Da
• NCBI Official Full Name: aquaporin 3, partial
• NCBI Official Synonym Full Names: aquaporin 3 (Gill blood group)
• NCBI Official Symbol: AQP3
• NCBI Official Synonym Symbols: GIL; AQP-3
• NCBI Protein Information: aquaporin-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group)
• UniProt Protein Name: Aquaporin-3
• UniProt Gene Name: AQP3
• UniProt Synonym Gene Names: AQP-3
• UniProt Entry Name: AQP3_HUMAN

NCBI Description

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]

Uniprot Description

AQP3: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Belongs to the MIP/aquaporin (TC 1.A.8) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, misc.; Membrane protein, multi-pass; Transporter; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; intercellular junction

Molecular Function: transporter activity; urea transmembrane transporter activity; water channel activity; glycerol channel activity

Biological Process: positive regulation of immune system process; glycerol transport; response to retinoic acid; cellular water homeostasis; water transport; excretion; odontogenesis; response to vitamin D; transport; renal water homeostasis; response to calcium ion; regulation of keratinocyte differentiation; transmembrane transport

Disease: Gil Blood Group