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Typical Testing Data/Standard Curve (for reference only) ()

Mouse 15-lipoxygenase (15-LO / LOX) ELISA Kit | 15-LO / LOX elisa kit

Mouse 15-lipoxygenase (15-LO / LOX) ELISA Kit

Gene Names
ALOX15; 12-LOX; 15LOX-1; 15-LOX-1
Reactivity
Mouse
Synonyms
15-lipoxygenase (15-LO / LOX); Mouse 15-lipoxygenase (15-LO / LOX) ELISA Kit; 15-LO / LOX elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
No cross-reaction with other factors.
Sequence Length
662
Samples
Mouse serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for 15-LO / LOX elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse 15-LO/LOX monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
246
Molecular Weight
74,804 Da
NCBI Official Full Name
15-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 15-lipoxygenase
NCBI Official Symbol
ALOX15
NCBI Official Synonym Symbols
12-LOX; 15LOX-1; 15-LOX-1
NCBI Protein Information
arachidonate 15-lipoxygenase; 15-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; arachidonate omega-6 lipoxygenase; arachidonate 12-lipoxygenase, leukocyte-type
UniProt Protein Name
Arachidonate 15-lipoxygenase
UniProt Gene Name
ALOX15
UniProt Synonym Gene Names
LOG15; 15-LOX; 15-LOX-1; 12-LOX
UniProt Entry Name
LOX15_HUMAN

Uniprot Description

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: Lipid Metabolism - linoleic acid; Oxidoreductase; Motility/polarity/chemotaxis; EC 1.13.11.33; EC 1.13.11.31; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; arachidonate 12-lipoxygenase activity; hepoxilin A3 synthase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; phosphatidylethanolamine biosynthetic process; leukotriene metabolic process; bone mineralization; positive regulation of actin filament polymerization; lipoxygenase pathway; negative regulation of adaptive immune response; arachidonic acid metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance

Research Articles on 15-LO / LOX

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Product Notes

The Mouse 15-LO / LOX alox15 (Catalog #AAA267034) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA267034 ELISA Kit recognizes Mouse 15-LO / LOX. It is sometimes possible for the material contained within the vial of "15-lipoxygenase (15-LO / LOX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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