Mouse Phospholipase A2 Membrane Associated ELISA Kit | Pla2g2a elisa kit
Mouse Phospholipase A2 Membrane Associated ELISA Kit
Gene Names
Pla2g2a; EF; Mom1; Pla2; sPLA2; sPla2-IIA
Reactivity
Mouse
Synonyms
Phospholipase A2 Membrane Associated; N/A; Mouse Phospholipase A2 Membrane Associated ELISA Kit; Pla2g2a elisa kit
Reactivity
Mouse
Assay Type
sandwich
Samples
cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Detection Target
Phospholipase A2 Membrane Associated
Immunogen Sequence
333
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Related Product Information for Pla2g2a elisa kit
This Mouse Phospholipase A2 Membrane Associated ELISA Kit is intended for quantitative detection of mouse PLA2G2A in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse PLA2G2A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLA2G2A has been precoated onto 96-well plates. Standards (Expression system for standard: sf21, Immunogen sequence: N22-C146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLA2G2A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 4 D3; 4 70.57 cM. Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains.
This mouse PLA2G2A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLA2G2A has been precoated onto 96-well plates. Standards (Expression system for standard: sf21, Immunogen sequence: N22-C146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLA2G2A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 4 D3; 4 70.57 cM. Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,145 Da
NCBI Official Full Name
phospholipase A2, membrane associated
NCBI Official Synonym Full Names
phospholipase A2, group IIA (platelets, synovial fluid)
NCBI Official Symbol
Pla2g2a
NCBI Official Synonym Symbols
EF; Mom1; Pla2; sPLA2; sPla2-IIA
NCBI Protein Information
phospholipase A2, membrane associated
UniProt Protein Name
Phospholipase A2, membrane associated
UniProt Gene Name
Pla2g2a
UniProt Synonym Gene Names
EF
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Product Notes
The Mouse Pla2g2a pla2g2a (Catalog #AAA266540) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA266540 ELISA Kit recognizes Mouse Pla2g2a. It is sometimes possible for the material contained within the vial of "Phospholipase A2 Membrane Associated, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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