Typical Testing Data/Standard Curve (for reference only)
Mouse 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit | 12-HETE elisa kit
Mouse 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit
Gene Names
GPR31; HETER; HETER1; 12-HETER
Reactivity
Mouse
Synonyms
12-Hydroxyeicosatetraenoic Acid (12-HETE); Mouse 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit; 12-HETE elisa kit
Reactivity
Mouse
Specificity
No cross-reaction with other factors.
Sequence Length
319
Samples
Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant 12-HETE concentration.
Detection Range
100 ng/ml-1.56 ng/ml
Sensitivity
Up to 0.5 ng/ml.
Assay Type
Sandwich
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for 12-HETE elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse 12-HETE monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,075 Da
NCBI Official Full Name
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
NCBI Official Synonym Full Names
G protein-coupled receptor 31
NCBI Official Symbol
GPR31
NCBI Official Synonym Symbols
HETER; HETER1; 12-HETER
NCBI Protein Information
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-HETE receptor; 12-(S)-HETE acid receptor; G-protein coupled receptor 31; probable G-protein coupled receptor 31; bA517H2.2 (G protein-coupled receptor 31); hydroxyeicosatetraenoic (HETE) acid receptor 1; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE) receptor
UniProt Protein Name
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
UniProt Gene Name
GPR31
UniProt Synonym Gene Names
12-(S)-HETE receptor; 12-HETER
UniProt Entry Name
GPR31_HUMAN
Uniprot Description
GPR31: High-affinity receptor for 12-(S)-hydroxy-5,8,10,14- eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B. Belongs to the G-protein coupled receptor 1 family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: 6q27
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway
Research Articles on 12-HETE
Similar Products
Product Notes
The Mouse 12-HETE gpr31 (Catalog #AAA265806) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA265806 ELISA Kit recognizes Mouse 12-HETE. It is sometimes possible for the material contained within the vial of "12-Hydroxyeicosatetraenoic Acid (12-HETE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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