Mouse urokinase plasminogen activator (uPA) ELISA Kit | uPA elisa kit

Mouse urokinase plasminogen activator (uPA) ELISA Kit

Cat. Num. AAA265656

• Gene Names: PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5
• Reactivity: Mouse
• Synonyms: urokinase plasminogen activator (uPA); Mouse urokinase plasminogen activator (uPA) ELISA Kit; uPA elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 411

• Samples: Mouse Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Tt4 Concentration
• Assay Type: Sandwich
• Detection Range: 200 ng/ml-3.12 ng/ml
• Sensitivity: 0.6 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for uPA elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse TT4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 56068043
• NCBI GeneID: 5328
• Molecular Weight: 46,908 Da
• NCBI Official Full Name: urokinase plasminogen activator, partial
• NCBI Official Synonym Full Names: plasminogen activator, urokinase
• NCBI Official Symbol: PLAU
• NCBI Official Synonym Symbols: ATF; QPD; UPA; URK; u-PA; BDPLT5
• NCBI Protein Information: urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary
• UniProt Protein Name: Urokinase-type plasminogen activator
• UniProt Gene Name: PLAU
• UniProt Synonym Gene Names: U-plasminogen activator; uPA
• UniProt Entry Name: UROK_HUMAN

NCBI Description

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

uPA: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Defects in PLAU are the cause of Quebec platelet disorder (QPD). QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.73; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: extracellular space; cell surface; focal adhesion; plasma membrane; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: regulation of cell adhesion mediated by integrin; fibrinolysis; regulation of smooth muscle cell migration; smooth muscle cell migration; response to hypoxia; regulation of receptor activity; chemotaxis; signal transduction; blood coagulation; proteolysis; regulation of cell proliferation

Disease: Quebec Platelet Disorder; Alzheimer Disease