Goat antiglomerular basement membrane (GBM) ELISA Kit | GBM elisa kit

Goat antiglomerular basement membrane (GBM) ELISA Kit

Cat. Num. AAA264270

• Gene Names: GCLM; GLCLR
• Reactivity: Goat
• Synonyms: antiglomerular basement membrane (GBM); Goat antiglomerular basement membrane (GBM) ELISA Kit; GBM elisa kit

• Reactivity: Goat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 274

• Samples: Goat Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Fsh Concentration
• Assay Type: Sandwich
• Detection Range: 100 mIU/ml-1.56 mIU/ml
• Sensitivity: 0.5 mIU/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GBM elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat FSH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504011
• NCBI GeneID: 2730
• NCBI Accession #: NP_002052.1
• NCBI GenBank Nucleotide #: NM_002061.2
• Molecular Weight: 30,727 Da
• NCBI Official Full Name: glutamate--cysteine ligase regulatory subunit
• NCBI Official Synonym Full Names: glutamate-cysteine ligase, modifier subunit
• NCBI Official Symbol: GCLM
• NCBI Official Synonym Symbols: GLCLR
• NCBI Protein Information: glutamate--cysteine ligase regulatory subunit; GCS light chain; GSC light chain; gamma-ECS regulatory subunit; glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; gamma-glutamylcysteine synthetase regulatory subunit; glutamate-cysteine ligase modifier subunit delta2 alternative splicing; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD)
• UniProt Protein Name: Glutamate--cysteine ligase regulatory subunit
• UniProt Gene Name: GCLM
• UniProt Synonym Gene Names: GLCLR
• UniProt Entry Name: GSH0_HUMAN

NCBI Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

Protein type: Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: protein heterodimerization activity; glutamate-cysteine ligase activity; enzyme regulator activity; glutamate-cysteine ligase catalytic subunit binding

Biological Process: response to drug; glutamate metabolic process; response to nitrosative stress; apoptotic mitochondrial changes; cysteine metabolic process; sulfur amino acid metabolic process; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; negative regulation of neuron apoptosis; regulation of mitochondrial depolarization; regulation of blood vessel size; positive regulation of glutamate-cysteine ligase activity

Disease: Myocardial Infarction, Susceptibility To