Human Aquaporin-1 (AQP1) ELISA Kit | AQP1 elisa kit

Human Aquaporin-1 (AQP1) ELISA Kit

Cat. Num. AAA262447

• Gene Names: AQP1; CO; CHIP28; AQP-CHIP
• Reactivity: Human
• Synonyms: Aquaporin-1 (AQP1); Human Aquaporin-1 (AQP1) ELISA Kit; AQP1 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 154

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: Up to 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for AQP1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human AQP1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 297307120
• NCBI GeneID: 358
• NCBI Accession #: NP_001171991.1
• NCBI GenBank Nucleotide #: NM_001185062.1
• Molecular Weight: 16,677 Da
• NCBI Official Full Name: aquaporin-1 isoform 4
• NCBI Official Synonym Full Names: aquaporin 1 (Colton blood group)
• NCBI Official Symbol: AQP1
• NCBI Official Synonym Symbols: CO; CHIP28; AQP-CHIP
• NCBI Protein Information: aquaporin-1; aquaporin-CHIP; urine water channel; channel-like integral membrane protein, 28-kDa; water channel protein for red blood cells and kidney proximal tubule; aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group)
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: AQP1
• UniProt Synonym Gene Names: CHIP28; AQP-1
• UniProt Entry Name: AQP1_HUMAN

NCBI Description

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear membrane; integral to plasma membrane; brush border membrane; basolateral plasma membrane; apical part of cell; cytoplasm; apical plasma membrane; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: protein binding; potassium channel activity; ephrin receptor binding; water transporter activity; ammonium transmembrane transporter activity; intracellular cGMP activated cation channel activity; glycerol transmembrane transporter activity; potassium ion transmembrane transporter activity; nitric oxide transporter activity; water channel activity; transmembrane transporter activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; cerebrospinal fluid secretion; wound healing; carbon dioxide transport; cellular response to stress; water transport; negative regulation of caspase activity; sensory perception of pain; pancreatic juice secretion; positive regulation of saliva secretion; odontogenesis; positive regulation of fibroblast proliferation; nitric oxide transport; hyperosmotic salinity response; bicarbonate transport; cellular homeostasis; multicellular organismal water homeostasis; transmembrane transport; lateral ventricle development; lipid digestion; potassium ion transport; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; renal water homeostasis; cell volume homeostasis; negative regulation of apoptosis

Disease: Blood Group--colton