Porcine beta-Thromboglobulin (beta-TG) ELISA Kit | beta-TG elisa kit

Porcine beta-Thromboglobulin (beta-TG) ELISA Kit

Cat. Num. AAA262430

• Gene Names: CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• Reactivity: Porcine
• Synonyms: beta-Thromboglobulin (beta-TG); Porcine beta-Thromboglobulin (beta-TG) ELISA Kit; Porcine b-Thromboglobulin (b-TG) ELISA Kit; beta-TG elisa kit

• Reactivity: Porcine
• Sequence Length: 99

• Samples: Porcine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for beta-TG elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine beta-TG monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 179580
• NCBI GeneID: 3576
• Molecular Weight: 11,338 Da
• NCBI Official Full Name: beta-thromboglobulin-like protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 8
• NCBI Official Symbol: CXCL8
• NCBI Official Synonym Symbols: IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• NCBI Protein Information: interleukin-8; emoctakin; interleukin 8; T-cell chemotactic factor; neutrophil-activating peptide 1; beta-thromboglobulin-like protein; granulocyte chemotactic protein 1; tumor necrosis factor-induced gene 1; alveolar macrophage chemotactic factor I; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; small inducible cytokine subfamily B, member 8; lymphocyte derived neutrophil activating peptide; lung giant cell carcinoma-derived chemotactic protein; beta endothelial cell-derived neutrophil activating peptide
• UniProt Protein Name: Interleukin-8
• UniProt Gene Name: CXCL8
• UniProt Synonym Gene Names: IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF
• UniProt Entry Name: IL8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]

Uniprot Description

IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; interleukin-8 receptor binding

Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; unfolded protein response; negative regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; signal transduction; chemotaxis; regulation of retroviral genome replication; induction of positive chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; receptor internalization; response to molecule of bacterial origin; immune response; angiogenesis; inflammatory response; cell motility; cell cycle arrest; embryonic gut development