Mouse Cyclophilin B (CYPB) ELISA Kit | CYPB elisa kit

Mouse Cyclophilin B (CYPB) ELISA Kit

Cat. Num. AAA262262

• Gene Names: PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
• Reactivity: Mouse
• Synonyms: Cyclophilin B (CYPB); Mouse Cyclophilin B (CYPB) ELISA Kit; CYPB elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 208

• Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant CYPB concentration.
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156ng/ml
• Sensitivity: The minimum detectable Mouse CYPB up to 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYPB elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse CYPB monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 181335
• NCBI GeneID: 5479
• Molecular Weight: 23,743 Da
• NCBI Official Full Name: cyclophilin B
• NCBI Official Synonym Full Names: peptidylprolyl isomerase B (cyclophilin B)
• NCBI Official Symbol: PPIB
• NCBI Official Synonym Symbols: OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase B; PPIase B; rotamase B; S-cyclophilin; cyclophilin-like protein; epididymis secretory protein Li 39
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase B
• Protein Family: Bifunctional cytochrome P450/NADPH--P450 reductase
• UniProt Gene Name: PPIB
• UniProt Synonym Gene Names: CYPB; PPIase B; SCYLP
• UniProt Entry Name: PPIB_HUMAN

NCBI Description

The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]

Uniprot Description

PPIB: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9). OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.

Protein type: Cyclophilin; EC 5.2.1.8; Isomerase; RNA-binding; Secreted; Secreted, signal peptide; Chaperone

Chromosomal Location of Human Ortholog: 15q21-q22

Cellular Component: smooth endoplasmic reticulum; focal adhesion; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; endoplasmic reticulum lumen; melanosome; nucleus

Molecular Function: collagen binding; protein binding; peptidyl-prolyl cis-trans isomerase activity; unfolded protein binding; protein complex binding; peptide binding

Biological Process: extracellular matrix organization and biogenesis; protein peptidyl-prolyl isomerization; protein stabilization; positive regulation of multicellular organism growth

Disease: Osteogenesis Imperfecta, Type Ix