Guinea Pig Fibrinopeptide A (FPA) ELISA Kit | FPA elisa kit

Guinea pig Fibrinopeptide A (FPA) ELISA Kit

Cat. Num. AAA261158

• Gene Names: FGA; Fib2
• Reactivity: Guinea Pig
• Synonyms: Fibrinopeptide A (FPA); Guinea pig Fibrinopeptide A (FPA) ELISA Kit; FPA elisa kit

• Reactivity: Guinea Pig
• Specificity: No cross-reaction with other factors.
• Sequence Length: 644

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant FPA concentration.
• Assay Type: Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: 0.5 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FPA elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig FPA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 11761629
• NCBI GeneID: 2243
• NCBI Accession #: NP_068657.1
• NCBI GenBank Nucleotide #: NM_021871.2
• Molecular Weight: 69,757 Da
• NCBI Official Full Name: fibrinogen alpha chain isoform alpha preproprotein
• NCBI Official Synonym Full Names: fibrinogen alpha chain
• NCBI Official Symbol: FGA
• NCBI Official Synonym Symbols: Fib2
• NCBI Protein Information: fibrinogen alpha chain; fibrinogen, A alpha polypeptide
• UniProt Protein Name: Fibrinogen alpha chain
• Protein Family: Flowering time control protein
• UniProt Gene Name: FGA
• UniProt Entry Name: FIBA_HUMAN

NCBI Description

The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus. [provided by RefSeq, Jul 2008]

Uniprot Description

FGA: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGA are a cause of congenital afibrinogenemia (CAFBN). This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha- dysfibrinogenemias. Defects in FGA are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: extracellular space; cell surface; fibrinogen complex; plasma membrane; extracellular region; cell cortex; vesicle; external side of plasma membrane

Molecular Function: protein binding, bridging; protein binding; cell adhesion molecule binding; structural molecule activity; receptor binding

Biological Process: protein polymerization; platelet activation; extracellular matrix organization and biogenesis; positive regulation of heterotypic cell-cell adhesion; platelet degranulation; cellular protein complex assembly; positive regulation of protein secretion; cell-matrix adhesion; positive regulation of vasoconstriction; innate immune response; signal transduction; blood coagulation; response to calcium ion; positive regulation of exocytosis

Disease: Amyloidosis, Familial Visceral; Afibrinogenemia, Congenital; Dysfibrinogenemia, Congenital