Bovine caenorhabditis elegans death gene (CED-3) ELISA Kit | CED-3 elisa kit

Bovine caenorhabditis elegans death gene(CED-3) ELISA Kit

Cat. Num. AAA2608935

• Reactivity: Bovine
• Synonyms: caenorhabditis elegans death gene (CED-3); Bovine caenorhabditis elegans death gene(CED-3) ELISA Kit; CED-3 elisa kit

• Reactivity: Bovine
• Sequence Length: 571

• Samples: Bovine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CED-3 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Bovine CED-3 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 62297305
• NCBI GeneID: 175643
• Molecular Weight: 25,290 Da
• NCBI Official Full Name: Cell death protein 4
• NCBI Official Symbol: ced-4
• NCBI Protein Information: Cell death protein 4
• UniProt Protein Name: Bcl-2-like protein 1
• Protein Family: Cell death protein
• UniProt Gene Name: BCL2L1
• UniProt Synonym Gene Names: BCL2L; BCLX; Bcl2-L-1
• UniProt Entry Name: B2CL1_HUMAN

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Apoptosis; Autophagy; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: centrosome; cytoplasm; mitochondrial outer membrane; mitochondrion

Molecular Function: BH3 domain binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding

Biological Process: apoptotic mitochondrial changes; cytokinesis; mitotic cell cycle checkpoint; negative regulation of apoptosis; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; release of cytochrome c from mitochondria; response to cytokine stimulus; suppression by virus of host apoptosis