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Typical Testing Data/Standard Curve (for reference only) ()

Human Serine/threonine-protein kinase PLK4, PLK4 ELISA Kit | PLK4 elisa kit

Human Serine/threonine-protein kinase PLK4, PLK4 ELISA Kit

Gene Names
PLK4; SAK; STK18
Reactivity
Human
Synonyms
Serine/threonine-protein kinase PLK4; PLK4; Human Serine/threonine-protein kinase PLK4; PLK4 ELISA Kit; PLK4 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Samples
Human serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for PLK4 elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human PLK4 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
108,972 Da
NCBI Official Full Name
serine/threonine-protein kinase PLK4 isoform 2
NCBI Official Synonym Full Names
polo-like kinase 4
NCBI Official Symbol
PLK4
NCBI Official Synonym Symbols
SAK; STK18
NCBI Protein Information
serine/threonine-protein kinase PLK4; PLK-4; Snk akin kinase; serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine protein kinase SAK; serine/threonine-protein kinase Sak
UniProt Protein Name
Serine/threonine-protein kinase PLK4
UniProt Gene Name
PLK4
UniProt Synonym Gene Names
SAK; STK18; PLK-4
UniProt Entry Name
PLK4_HUMAN

NCBI Description

This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

PLK4: a kinase of the PLK family. Localizes to centrosomes and the cleavage furrow during cytokinesis. Interacts with the protein kinase Tec.

Protein type: Kinase, protein; Nucleolus; Protein kinase, Other; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: centriole; centrosome; nucleolus; cytosol; cleavage furrow

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: organelle organization and biogenesis; positive regulation of centriole replication; centriole replication; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation

Disease: Microcephaly And Chorioretinopathy, Autosomal Recessive, 2

Research Articles on PLK4

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Product Notes

The Human PLK4 plk4 (Catalog #AAA2602717) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2602717 ELISA Kit recognizes Human PLK4. It is sometimes possible for the material contained within the vial of "Serine/threonine-protein kinase PLK4, PLK4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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