Human G antigen family C member 1, PAGE4 ELISA Kit | PAGE4 elisa kit

Human G antigen family C member 1, PAGE4 ELISA Kit

Cat. Num. AAA2602653

• Gene Names: PAGE4; JM27; JM-27; CT16.7; GAGE-9; GAGEC1; PAGE-1; PAGE-4
• Reactivity: Human
• Synonyms: G antigen family C member 1; PAGE4; Human G antigen family C member 1; PAGE4 ELISA Kit; PAGE4 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for PAGE4 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human PAGE4 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 5901986
• NCBI GeneID: 9506
• NCBI Accession #: NP_008934.1
• NCBI GenBank Nucleotide #: NM_007003.3
• UniProt Accession #: O60829; Q6IBI1; B2R529; D3DX68
• Molecular Weight: 11,153 Da
• NCBI Official Full Name: P antigen family member 4
• NCBI Official Synonym Full Names: P antigen family, member 4 (prostate associated)
• NCBI Official Symbol: PAGE4
• NCBI Official Synonym Symbols: JM27; JM-27; CT16.7; GAGE-9; GAGEC1; PAGE-1; PAGE-4
• NCBI Protein Information: P antigen family member 4; g antigen family C member 1; prostate-associated gene protein 4
• UniProt Protein Name: P antigen family member 4
• Protein Family: P antigen family
• UniProt Gene Name: PAGE4
• UniProt Synonym Gene Names: GAGEC1; JM27; PAGE-4
• UniProt Entry Name: PAGE4_HUMAN

NCBI Description

This gene is a member of the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in prostate and prostate cancer. It is also expressed in other male and female reproductive tissues including testis, fallopian tube, uterus, and placenta, as well as in testicular cancer and uterine cancer. The protein encoded by this gene shares sequence similarity with other GAGE/PAGE proteins, and also belongs to a family of CT (cancer-testis) antigens. The protein may play a role in benign and malignant prostate diseases. A related pseudogene is located on chromosome 7. [provided by RefSeq, Aug 2013]

Uniprot Description

PAGE4: a member of the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. strongly expressed in prostate and prostate cancer, but is also expressed in other male and female reproductive tissues including testis, fallopian tube, uterus, and placenta, as well as in testicular cancer and uterine cancer. The protein encoded by this gene shares sequence similarity with other GAGE/PAGE proteins, and also belongs to a family of CT (cancer-testis) antigens. [provided by RefSeq, Jul 2008]

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xp11.23