Mouse Aldose Reductase (AR) ELISA Kit | AR elisa kit

Mouse Aldose Reductase (AR) ELISA Kit

Cat. Num. AAA2601930

• Gene Names: AKR1B1; AR; ADR; ALR2; ALDR1
• Reactivity: Mouse
• Synonyms: Aldose Reductase (AR); Mouse Aldose Reductase (AR) ELISA Kit; AR elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 316

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant AR concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AR elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse AR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502049
• NCBI GeneID: 231
• NCBI Accession #: NP_001619.1
• NCBI GenBank Nucleotide #: NM_001628.2
• Molecular Weight: 35,853 Da
• NCBI Official Full Name: aldose reductase
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member B1 (aldose reductase)
• NCBI Official Symbol: AKR1B1
• NCBI Official Synonym Symbols: AR; ADR; ALR2; ALDR1
• NCBI Protein Information: aldose reductase; aldehyde reductase 1; low Km aldose reductase; Lii5-2 CTCL tumor antigen; aldo-keto reductase family 1 member B1
• UniProt Protein Name: Aldose reductase
• Protein Family: Allatostatin
• UniProt Gene Name: AKR1B1
• UniProt Synonym Gene Names: ALDR1; AR
• UniProt Entry Name: ALDR_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

Uniprot Description

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - galactose; Lipid Metabolism - glycerolipid; Oxidoreductase; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 1.1.1.21

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol

Molecular Function: glyceraldehyde oxidoreductase activity; electron carrier activity; aldo-keto reductase activity; aldehyde reductase activity

Biological Process: steroid metabolic process; carbohydrate metabolic process; response to stress; C21-steroid hormone biosynthetic process