Antigen Coating Buffer, 5X Blocking Buffer
Antigen Coating Buffer, 5X
Synonyms
Antigen Coating Buffer; 5X; 5X blocking buffer
Form/Format
Supplied at 5X.
Application Notes
1. Dilute Antigen Coating Buffer, 5X 1:5 using deionized water and mix for 15 minutes. As Antigen Coating Buffer is a 5X concentrate, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm the buffer until all crystals are dissolved. Do not let it boil.
2. Dilute your antigen into the coating buffer. Optimal coating concentration varies significantly from 0.2 ug/mL to 10 ug/mL.
3. Let the solution stir 10-15 minutes and pipette onto the plate. Optimal coating volume generally ranges from 50-200 uL per well. ICT recommends coating antibodies onto Immulon II HB plates.
4. Once added to the plate, incubate the coating solution from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering.
5. After incubation, dump or aspirate the coating solution out of the wells.
6. Wash the plate twice with ICT's ELISA Wash Buffer.
7. Aspirate and pipette one of ICT's Blocking Buffers onto the plate at a higher volume than the coating solution (300-400 uL per well).
8. Once added to the plate, incubate the block buffer from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering.
9. Aspirate the block buffer.
10. The assay can be run at this point, or the plate can be dried and packaged for later use.
11. Dry the plate by letting it sit in the fume hood covered with foil overnight, or dry in a drying chamber under vacuum from 3-6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant and store at 2-8 degree C protected from light.
2. Dilute your antigen into the coating buffer. Optimal coating concentration varies significantly from 0.2 ug/mL to 10 ug/mL.
3. Let the solution stir 10-15 minutes and pipette onto the plate. Optimal coating volume generally ranges from 50-200 uL per well. ICT recommends coating antibodies onto Immulon II HB plates.
4. Once added to the plate, incubate the coating solution from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering.
5. After incubation, dump or aspirate the coating solution out of the wells.
6. Wash the plate twice with ICT's ELISA Wash Buffer.
7. Aspirate and pipette one of ICT's Blocking Buffers onto the plate at a higher volume than the coating solution (300-400 uL per well).
8. Once added to the plate, incubate the block buffer from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering.
9. Aspirate the block buffer.
10. The assay can be run at this point, or the plate can be dried and packaged for later use.
11. Dry the plate by letting it sit in the fume hood covered with foil overnight, or dry in a drying chamber under vacuum from 3-6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant and store at 2-8 degree C protected from light.
Contains
No proteins
Preparation and Storage
Store at room temperature or 2-8 degree C.
Related Product Information for Antigen Coating Buffer, 5X blocking buffer
Description: Antigen Coating Buffer maximizes the adsorption of antigens onto polystyrene plates and stabilizes their structure to enhance specific signal, save reagents, and extend the shelf life of coated plates.
Background: Antigen Coating Buffer, 5X maximizes the adsorption of antigens onto polystyrene plates for use in an indirect ELISA. By stabilizing the tertiary structure of the antigen, Antigen Coating Buffer helps the antigen retain its binding reactivity with the target antibody in the sample. This product is best for coating proteins ranging from 6 kDa to > 200 kDa onto polystyrene plates.
Stabilizing the coated antigen allows for better analyte detection and enhances the specific signal of the assay. By generating a higher positive signal, a lower concentration of coating antigen may be used, saving valuable reagents.
Under proper storage conditions, coated plates may last for years, enabling the manufacture of large batches for inclusion in test kits. This also proves useful in non-manufacturing settings, where large batches of plates may be prepared at once to be stored for future experiments.
Background: Antigen Coating Buffer, 5X maximizes the adsorption of antigens onto polystyrene plates for use in an indirect ELISA. By stabilizing the tertiary structure of the antigen, Antigen Coating Buffer helps the antigen retain its binding reactivity with the target antibody in the sample. This product is best for coating proteins ranging from 6 kDa to > 200 kDa onto polystyrene plates.
Stabilizing the coated antigen allows for better analyte detection and enhances the specific signal of the assay. By generating a higher positive signal, a lower concentration of coating antigen may be used, saving valuable reagents.
Under proper storage conditions, coated plates may last for years, enabling the manufacture of large batches for inclusion in test kits. This also proves useful in non-manufacturing settings, where large batches of plates may be prepared at once to be stored for future experiments.
Product Categories/Family for Antigen Coating Buffer, 5X blocking buffer
Similar Products
Product Notes
The Antigen Coating Buffer, 5X (Catalog #AAA258387) is a Blocking Buffer and is intended for research purposes only. The product is available for immediate purchase. 1. Dilute Antigen Coating Buffer, 5X 1:5 using deionized water and mix for 15 minutes. As Antigen Coating Buffer is a 5X concentrate, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm the buffer until all crystals are dissolved. Do not let it boil. 2. Dilute your antigen into the coating buffer. Optimal coating concentration varies significantly from 0.2 ug/mL to 10 ug/mL. 3. Let the solution stir 10-15 minutes and pipette onto the plate. Optimal coating volume generally ranges from 50-200 uL per well. ICT recommends coating antibodies onto Immulon II HB plates. 4. Once added to the plate, incubate the coating solution from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering. 5. After incubation, dump or aspirate the coating solution out of the wells. 6. Wash the plate twice with ICT's ELISA Wash Buffer. 7. Aspirate and pipette one of ICT's Blocking Buffers onto the plate at a higher volume than the coating solution (300-400 uL per well). 8. Once added to the plate, incubate the block buffer from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering. 9. Aspirate the block buffer. 10. The assay can be run at this point, or the plate can be dried and packaged for later use. 11. Dry the plate by letting it sit in the fume hood covered with foil overnight, or dry in a drying chamber under vacuum from 3-6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant and store at 2-8 degree C protected from light. Researchers should empirically determine the suitability of the Antigen Coating Buffer, 5X for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Antigen Coating Buffer, 5X, Blocking Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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