Sample Diluent Optimization Pack Diluent
Sample Diluent Optimization Pack: All Three 100mL SDs
Synonyms
Sample Diluent Optimization Pack; Sample Diluent Optimization Pack: All Three 100mL SDs; Sample Diluent Optimization Pack diluent
Form/Format
Supplied at 1X.
Application Notes
1. To quickly determine which Sample Diluent formulation is best for the assay, all three Sample Diluents can be run in parallel on the same IgG or antigen-coated ELISA plate.
2. Determine the initial dynamic analyte range of the ELISA curves that were prepared in each of the three different Sample Diluent types.
3. Make test spikes into serum samples.
4. Read the spiked serum sample wells on each of the three SD-associated standard curves to see which Sample Diluent gives the best approximation of the spike concentration that was added to the serum samples.
5. In cases where a high level of matrix complexity interference is detected, inclusion of an additional Assay Diluent reagent may also be required to bring the calibrator well antibody-to-antigen binding kinetics closer to the sample well binding kinetics.
2. Determine the initial dynamic analyte range of the ELISA curves that were prepared in each of the three different Sample Diluent types.
3. Make test spikes into serum samples.
4. Read the spiked serum sample wells on each of the three SD-associated standard curves to see which Sample Diluent gives the best approximation of the spike concentration that was added to the serum samples.
5. In cases where a high level of matrix complexity interference is detected, inclusion of an additional Assay Diluent reagent may also be required to bring the calibrator well antibody-to-antigen binding kinetics closer to the sample well binding kinetics.
pH
7.4 at 1X
Contains
General Serum Diluent-Mammalian proteins; Plasma Sample Diluent-Mammalian proteins; Neptune Sample Diluent-Non-mammalian proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Sample Diluent Optimization Pack diluent
Description: Sample Diluent Optimization Pack provides three 100 mL bottles of Sample Diluent formulations (General Serum Diluent, Plasma Sample Diluent, and Neptune Sample Diluent) for an economical and fast method of selecting the best Sample Diluent for a particular assay.
Background: When developing any new ELISA, it is often helpful to compare a number of different sample diluent formulations to better match the matrix complexity present in the sample wells with the standard curve calibrator matrix wells. Most of the time invested in developing a new ELISA/EIA/RIA will involve selecting the proper calibrator matrix that matches most closely to the often very complex biological sample matrix that is being evaluated. Comparing three different sample/calibrator diluent formulations on the same IgG or antigen-coated ELISA plate makes it is easy to determine which diluent will best equalize the calibrator well antibody-binding kinetics with the biological sample/ dilution well antibody-binding kinetics. The Sample Diluent Optimization Pack provides 100 mL of each of our three Sample Diluent formulations to begin optimizing the sample diluent portion of the assay development process.
Background: When developing any new ELISA, it is often helpful to compare a number of different sample diluent formulations to better match the matrix complexity present in the sample wells with the standard curve calibrator matrix wells. Most of the time invested in developing a new ELISA/EIA/RIA will involve selecting the proper calibrator matrix that matches most closely to the often very complex biological sample matrix that is being evaluated. Comparing three different sample/calibrator diluent formulations on the same IgG or antigen-coated ELISA plate makes it is easy to determine which diluent will best equalize the calibrator well antibody-binding kinetics with the biological sample/ dilution well antibody-binding kinetics. The Sample Diluent Optimization Pack provides 100 mL of each of our three Sample Diluent formulations to begin optimizing the sample diluent portion of the assay development process.
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Product Notes
The Sample Diluent Optimization Pack (Catalog #AAA258064) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. 1. To quickly determine which Sample Diluent formulation is best for the assay, all three Sample Diluents can be run in parallel on the same IgG or antigen-coated ELISA plate. 2. Determine the initial dynamic analyte range of the ELISA curves that were prepared in each of the three different Sample Diluent types. 3. Make test spikes into serum samples. 4. Read the spiked serum sample wells on each of the three SD-associated standard curves to see which Sample Diluent gives the best approximation of the spike concentration that was added to the serum samples. 5. In cases where a high level of matrix complexity interference is detected, inclusion of an additional Assay Diluent reagent may also be required to bring the calibrator well antibody-to-antigen binding kinetics closer to the sample well binding kinetics. Researchers should empirically determine the suitability of the Sample Diluent Optimization Pack for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sample Diluent Optimization Pack, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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