Hoechst 33342 Blue Nuclear DNA dye Reagent
Hoechst 33342 blue nuclear DNA dye
Applications
Flow Cytometer, Fluorescence Microscope
Synonyms
Hoechst 33342 Blue Nuclear DNA dye; Hoechst 33342 blue nuclear DNA dye; Hoechst 33342 Fluorescent Nucleic Acid Stain; Hoechst 33342 Blue Nuclear DNA dye reagent
Form/Format
Supplied at 200 ug/mL.
Applicable Applications for Hoechst 33342 Blue Nuclear DNA dye reagent
Flow Cytometer, Fluorescence Microscope
Application Notes
1. Add Hoechst 33342 to the cell sample media at 0.5% v/v. For example, add 1.5 uL Hoechst to 300 uL of cells.
2. Incubate 10-20 minutes at room temperature.
3. Visualize with a fluorescence microscope by using a UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm.
4. Alternatively, cells may be analyzed with a flow cytometer using a UV excitation source.
5. When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461.
2. Incubate 10-20 minutes at room temperature.
3. Visualize with a fluorescence microscope by using a UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm.
4. Alternatively, cells may be analyzed with a flow cytometer using a UV excitation source.
5. When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461.
pH
4.0 + 1.0
Excitation/Emission
350 nm / 480 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Hoechst 33342 Blue Nuclear DNA dye reagent
Description: Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pyknotic nuclei in apoptotic cells and for cell cycle studies.
Background: Hoechst 33342 is a popular cell-permeant, blue fluorescent nuclear stain. It is used to visualize the nuclei of living or fixed cells and tissues and is often used to distinguish condensed, pyknotic nuclei in apoptotic cells. Hoechst 33342 emits blue fluorescence when bound to double stranded DNA. It is slightly more membrane permeant than the Hoechst 33258 analog. Hoechst 33342 may be used to identify healthy or apoptotic nuclear morphology and for cell cycle studies.
Background: Hoechst 33342 is a popular cell-permeant, blue fluorescent nuclear stain. It is used to visualize the nuclei of living or fixed cells and tissues and is often used to distinguish condensed, pyknotic nuclei in apoptotic cells. Hoechst 33342 emits blue fluorescence when bound to double stranded DNA. It is slightly more membrane permeant than the Hoechst 33258 analog. Hoechst 33342 may be used to identify healthy or apoptotic nuclear morphology and for cell cycle studies.
Product Categories/Family for Hoechst 33342 Blue Nuclear DNA dye reagent
Similar Products
Product Notes
The Hoechst 33342 Blue Nuclear DNA dye (Catalog #AAA258061) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hoechst 33342 Blue Nuclear DNA dye can be used in a range of immunoassay formats including, but not limited to, Flow Cytometer, Fluorescence Microscope. 1. Add Hoechst 33342 to the cell sample media at 0.5% v/v. For example, add 1.5 uL Hoechst to 300 uL of cells. 2. Incubate 10-20 minutes at room temperature. 3. Visualize with a fluorescence microscope by using an UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm. 4. Alternatively, cells may be analyzed with a flow cytometer using an UV excitation source. 5. When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461. Researchers should empirically determine the suitability of the Hoechst 33342 Blue Nuclear DNA dye for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hoechst 33342 Blue Nuclear DNA dye, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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