Human CEA (Carcinoembryonic Antigen) ELISA Kit | CEA elisa kit

QuicKey Pro Human CEA (Carcinoembryonic Antigen) ELISA Kit

Cat. Num. AAA2568098

• Gene Names: CEACAM5; CEA; CD66e
• Reactivity: Human
• Synonyms: CEA (Carcinoembryonic Antigen); QuicKey Pro Human CEA (Carcinoembryonic Antigen) ELISA Kit; CEA; Carcinoembryonic Antigen; CEA elisa kit

• Reactivity: Human
• Specificity: This kit recognizes CEA in samples. No significant cross-reactivity or interference between CEA and analogues was observed.; This ELISA kit applies to the in vitro quantitative determination of CEA concentrations in serum and plasma.

• Assay Type: Sandwich ELISA
• Detection Method: Colormetric
• Tested Sample Types: Serum and plasma
• Detection Range: 0.78-50ng/mL
• Sensitivity: 0.47ng/mL
• Precision: Both intra-CV and inter-CV are < 10%.
• Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CEA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CEA are added to the micro ELISA plate wells. Human CEA in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to CEA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450+/-2nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.
• Preparation and Storage: An unopened kit can be stored at 2-8? for 6 months. After test, the unused wells and reagents should be stored according to the table/manual.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 317373456
• NCBI GeneID: 1048
• UniProt Accession #: P06731; H9KVA7
• Molecular Weight: 76,795 Da
• NCBI Official Full Name: Carcinoembryonic antigen-related cell adhesion molecule 5
• NCBI Official Synonym Full Names: carcinoembryonic antigen-related cell adhesion molecule 5
• NCBI Official Symbol: CEACAM5
• NCBI Official Synonym Symbols: CEA; CD66e
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 5
• Protein Family: Colicin
• UniProt Gene Name: CEACAM5
• UniProt Synonym Gene Names: CEA; CEA
• UniProt Entry Name: CEAM5_HUMAN

NCBI Description

Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]

Uniprot Description

CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis