COVID 19 NSP8 Protein Coronavirus Recombinant Protein | COVID-19 recombinant protein
Recombinant 2019-nCoV NSP8 Protein (His Tag)
Purity
>95% as determined by reducing SDS-PAGE.
Synonyms
COVID 19 NSP8 Protein Coronavirus; Recombinant 2019-nCoV NSP8 Protein (His Tag); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; COVID-19 recombinant protein
Host
E Coli
Purity/Purification
>95% as determined by reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 um Filtered Solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.
Sequence Positions
Ala1-Gln198
Sequence Length
198
Species
Virus
Endotoxin
< 1.0 EU per ug as determined by the LAL method.
Fusion Tag
C-6His
Preparation and Storage
Store at < -20 degree C, stable for 6 months. Please minimize freeze-thaw cycles.
Related Product Information for COVID-19 recombinant protein
Protein Construction: Recombinant 2019-nCoV NSP8 is produced by our E.coli expression system and the target gene encoding Ala1-Gln198 is expressed with a 6His tag at the C-terminus.
Background: Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein, The nsp8 protein has been shown to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis. It forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. Nsp8 was shown to have RNA-dependent RNA polymerase (RdRp) activity that could be involved in producing primers utilized by nsp12 which is normally accepted to be the RdRp for SARS-CoV.
Background: Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein, The nsp8 protein has been shown to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis. It forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. Nsp8 was shown to have RNA-dependent RNA polymerase (RdRp) activity that could be involved in producing primers utilized by nsp12 which is normally accepted to be the RdRp for SARS-CoV.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular Mass: 25 kDa
Actual Protein Molecular Mass: 25 kDa
Actual Protein Molecular Mass: 25 kDa
NCBI Official Full Name
nsp8
Similar Products
Product Notes
The COVID-19 (Catalog #AAA2563856) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ala1-Gln198. It is sometimes possible for the material contained within the vial of "COVID 19 NSP8 Protein Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
