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Western Blot (WB) (Western blot analysis of 293T Hela HepG2 and Jurkat cell lysates using MRPL42 Polyclonal Antibody at dilution of 1:650)

Rabbit anti-Human, Rat MRPL42 Polyclonal Antibody | anti-MRPL42 antibody

MRPL42 Polyclonal Antibody

Gene Names
MRPL42; L31MT; L42MT; S32MT; MRPL31; MRPS32; PTD007; RPML31; HSPC204; MRP-L31; MRP-L42; MRP-S32
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Antigen Affinity Purification
Synonyms
MRPL42; Polyclonal Antibody; MRPL42 Polyclonal Antibody; 28S ribosomal protein S32; 39S ribosomal protein L31; 39S ribosomal protein L42; HSPC204; L31mt; L42mt; Mitochondrial; Mitochondrial ribosomal protein L42; Mitochondrial ribosomal protein S32; MRP L31; MRP L42; MRP S32; MRP-L31; MRP-L42; MRP-S32; MRPL31; Mrpl42; MRPS32; PTD007; RM42; RPML31; S32mt; anti-MRPL42 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Concentration
1.02mg/mL (varies by lot)
Sequence Length
142
Applicable Applications for anti-MRPL42 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA: 1:5000-1:10000
Immunogen
Fusion protein of human MRPL42
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 293T Hela HepG2 and Jurkat cell lysates using MRPL42 Polyclonal Antibody at dilution of 1:650)

Western Blot (WB) (Western blot analysis of 293T Hela HepG2 and Jurkat cell lysates using MRPL42 Polyclonal Antibody at dilution of 1:650)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer tissue using MRPL42 Polyclonal Antibody at dilution of 1:60(×200))

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using MRPL42 Polyclonal Antibody at dilution of 1:60(×200))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using MRPL42 Polyclonal Antibody at dilution of 1:60(×200))

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using MRPL42 Polyclonal Antibody at dilution of 1:60(×200))
Related Product Information for anti-MRPL42 antibody
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.
Product Categories/Family for anti-MRPL42 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17 kDa
NCBI Official Full Name
39S ribosomal protein L42, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L42
NCBI Official Symbol
MRPL42
NCBI Official Synonym Symbols
L31MT; L42MT; S32MT; MRPL31; MRPS32; PTD007; RPML31; HSPC204; MRP-L31; MRP-L42; MRP-S32
NCBI Protein Information
39S ribosomal protein L42, mitochondrial
UniProt Protein Name
39S ribosomal protein L42, mitochondrial
Protein Family
UniProt Gene Name
MRPL42
UniProt Synonym Gene Names
MRPL31; MRPS32; RPML31; L42mt; MRP-L42; MRP-S32; S32mt; L31mt; MRP-L31
UniProt Entry Name
RM42_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq, May 2011]

Uniprot Description

MRPL42: Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins. Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.

Protein type: Ribosomal; Mitochondrial

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial small ribosomal subunit; plasma membrane

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; translation; organelle organization and biogenesis

Research Articles on MRPL42

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Product Notes

The MRPL42 mrpl42 (Catalog #AAA2562942) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRPL42 Polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MRPL42 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA: 1:5000-1:10000. Researchers should empirically determine the suitability of the MRPL42 mrpl42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPL42, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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