IF (Immunofluorescence)
(Immunofluorescence staining of NUDT10 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUDT10 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human NUDT10 Polyclonal Antibody | anti-NUDT10 antibody
Anti-NUDT10 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
NUDT10, Antibody; Anti-NUDT10 Antibody, Rabbit Polyclonal; NUDT10 Antibody, Rabbit PAb, Antigen Affinity Purified; nudix hydrolase 10; Anti-APS2 Antibody; Anti-DIPP3-alpha Antibody; Anti-DIPP3a Antibody; anti-NUDT10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NUDT10
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-NUDT10 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
E Coli-derived Human NUDT10 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human NUDT10 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of NUDT10 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUDT10 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of NUDT10 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUDT10 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohistochemistry)
(Immunochemical staining of human NUDT10 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human NUDT10 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-NUDT10 antibody
NUDT10 (Nudix Hydrolase 10) is a Protein Coding gene. It is located on Xp11.22. This gene is a member of the nudix (nucleoside diphosphate linked moiety X)-type motif-containing family. The encoded protein is a phosphohydrolase and may regulate the turnover of diphosphoinositol polyphosphates. The turnover of these high-energy diphosphoinositol polyphosphates represents a molecular switching activity with important regulatory consequences. Molecular switching by diphosphoinositol polyphosphates may contribute to the regulation of intracellular trafficking. In some populations, putative prostate cancer susceptibility alleles have been identified for this gene. Diseases associated with NUDT10 include Autoimmune Polyendocrine Syndrome, Type Ii, and Adrenal Cortical Hypofunction. Among its related pathways are Metabolism and Inositol phosphate metabolism (REACTOME).
References
Hidaka K, et al. (2002) An adjacent pair of human nudt genes on chromosome x are preferentially expressed in testis and encode two new isoforms of diphosphoinositol polyphosphate phosphohydrolase. J Biol Chem 277 (36): 32730-32738.Gudmundsson J, et al. (2008) Common sequence variants on 2p15 and xp11.22 confer susceptibility to prostate cancer. Nat Genet 40 (3): 281-283.Fisher DI, et al. (2002) Nudix hydrolases that degrade dinucleoside and diphosphoinositol polyphosphates also have 5-phosphoribosyl 1-pyrophosphate (prpp) pyrophosphatase activity that generates the glycolytic activator ribose 1, 5-bisphosphate. J Biol Chem 277 (49): 47313-47317.
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Product Notes
The NUDT10 (Catalog #AAA256255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NUDT10 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUDT10 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NUDT10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUDT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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