Western Blot (WB)
(Western blot analysis of Mouse brain tissue and Rat brain tissue lysates using SLC25A27 Polyclonal Antibody at dilution of 1:350)
Rabbit SLC25A27 Polyclonal Antibody | anti-SLC25A27 antibody
SLC25A27 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Antigen Affinity Purification
Synonyms
SLC25A27; Polyclonal Antibody; SLC25A27 Polyclonal Antibody; Mitochondrial uncoupling protein 4; Solute carrier family 25 member 27; UCP 4; UCP4; Uncoupling protein 4; anti-SLC25A27 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Concentration
0.9mg/mL (varies by lot)
Sequence Length
300
Applicable Applications for anti-SLC25A27 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:20-1:100
ELISA: 1:5000-1:10000
IHC: 1:20-1:100
ELISA: 1:5000-1:10000
Immunogen
Synthetic peptide of human SLC25A27
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Mouse brain tissue and Rat brain tissue lysates using SLC25A27 Polyclonal Antibody at dilution of 1:350)
Western Blot (WB)
(Western blot analysis of Mouse brain tissue and Rat brain tissue lysates using SLC25A27 Polyclonal Antibody at dilution of 1:350)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using SLC25A27 Polyclonal Antibody at dilution of 1:30(×200))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using SLC25A27 Polyclonal Antibody at dilution of 1:30(×200))
Related Product Information for anti-SLC25A27 antibody
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-SLC25A27 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36 kDa
NCBI Official Full Name
mitochondrial uncoupling protein 4 isoform 2
UniProt Protein Name
Mitochondrial uncoupling protein 4
UniProt Gene Name
SLC25A27
UniProt Synonym Gene Names
UCP4; UCP 4
UniProt Entry Name
UCP4_HUMAN
Uniprot Description
SLC25A27: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. May play a role in thermoregulatory heat production and metabolism in brain. Belongs to the mitochondrial carrier family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 6p12.3
Cellular Component: mitochondrion; cell soma; apical part of cell; mitochondrial inner membrane; integral to membrane
Biological Process: generation of precursor metabolites and energy; transport; positive regulation of cell proliferation; regulation of glucose import; reduction of mitochondrial calcium ion concentration; inner ear development; negative regulation of apoptosis
Similar Products
Product Notes
The SLC25A27 slc25a27 (Catalog #AAA2559809) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC25A27 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLC25A27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:20-1:100 ELISA: 1:5000-1:10000. Researchers should empirically determine the suitability of the SLC25A27 slc25a27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC25A27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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