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Flow Cytometry (FC/FACS) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD4 Monoclonal Antibody(FITC Conjugated) and Anti-Mouse CD25 Monoclonal Antibody(PE/Cyanine5 Conjugated))

Rat anti-Mouse CD25 Monoclonal Antibody | anti-CD25 antibody

Anti-Mouse CD25 Monoclonal Antibody (PE/Cyanine5 Conjugated) [PC-61.5.3]

Gene Names
Il2ra; CD25; Il2r; Ly-43
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD25; Monoclonal Antibody; Anti-Mouse CD25 Monoclonal Antibody (PE/Cyanine5 Conjugated) [PC-61.5.3]; Interleukin-2 receptor subunit alpha; IL2RA; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; anti-CD25 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
PC-61.5.3
Form/Format
PBS with 0.05% Proclin300, 1% BSA
Concentration
0.5 mg/mL (varies by lot)
Sequence Length
268
Applicable Applications for anti-CD25 antibody
Flow Cytometry (FC/FACS)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugation
PE/Cyanine5
Preparation and Storage
Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse CD4 Monoclonal Antibody(FITC Conjugated) and Anti-Mouse CD25 Monoclonal Antibody(PE/Cyanine5 Conjugated))

Flow Cytometry (FC/FACS) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD4 Monoclonal Antibody(FITC Conjugated) and Anti-Mouse CD25 Monoclonal Antibody(PE/Cyanine5 Conjugated))
Related Product Information for anti-CD25 antibody
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Ralpha, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rbeta) and CD132(common gamma chain), CD25 forms the high affinity signaling IL-2 receptor.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Interleukin-2 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha chain
NCBI Official Symbol
Il2ra
NCBI Official Synonym Symbols
CD25; Il2r; Ly-43
NCBI Protein Information
interleukin-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Gene Name
Il2ra
UniProt Synonym Gene Names
Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_MOUSE

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; inflammatory response to antigenic stimulus; negative regulation of T cell proliferation; positive regulation of T cell differentiation; negative regulation of inflammatory response; T cell homeostasis; positive regulation of T cell proliferation; immune response; activated T cell apoptosis

Research Articles on CD25

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Product Notes

The CD25 il2ra (Catalog #AAA2558683) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD25 Monoclonal Antibody (PE/Cyanine5 Conjugated) [PC-61.5.3] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD25 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Researchers should empirically determine the suitability of the CD25 il2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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