Rat anti-Human, Mouse CD44 Monoclonal Antibody | anti-CD44 antibody

Anti-Mouse/Human CD44 Monoclonal Antibody (FITC Conjugated) [IM7]

Cat. Num. AAA2558657

• Gene Names: Cd44; Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD44; Monoclonal Antibody; Anti-Mouse/Human CD44 Monoclonal Antibody (FITC Conjugated) [IM7]; CD44 antigen; CDw44; Epican; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; LHR; MDU2; MDU3; MIC4; anti-CD44 antibody

• Host: Rat
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: IM7
• Form/Format: PBS with 0.05% Proclin300, 1% BSA
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 365

• Applicable Applications for anti-CD44 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 10⁶ cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
• Conjugation: FITC
• Preparation and Storage: Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse/Human CD44 Monoclonal Antibody(FITC Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD44 antibody

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

NCBI and Uniprot Product Information

• NCBI GI #: 85540468
• NCBI GeneID: 12505
• NCBI Accession #: NP_001034240.1
• NCBI GenBank Nucleotide #: NP_001034240.1
• UniProt Accession #: P15379; P16070
• NCBI Official Full Name: CD44 antigen isoform c
• NCBI Official Synonym Full Names: CD44 antigen
• NCBI Official Symbol: Cd44
• NCBI Official Synonym Symbols: Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
• NCBI Protein Information: CD44 antigen
• UniProt Protein Name: CD44 antigen
• Protein Family: CD44 antigen
• UniProt Gene Name: Cd44
• UniProt Synonym Gene Names: Ly-24; ECMR-III; Ly-24; PGP-1; PGP-I
• UniProt Entry Name: CD44_MOUSE

Uniprot Description

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis (). Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (PubMed:25065622).