RHEB/RHEB2 recombinant protein

Recombinant Human RHEB/RHEB2 Protein (N-GST)

Cat. Num. AAA2557154

• Gene Names: RHEB; RHEB2
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: RHEB/RHEB2; Recombinant Human RHEB/RHEB2 Protein (N-GST); GTP-Binding Protein Rheb; Ras Homolog Enriched in Brain; RHEB; RHEB2; RHEB/RHEB2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM Tris, 10mM GSH, pH 8.0.
• Sequence: Met1-Met184
• Sequence Length: 184

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: N-GST
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for RHEB/RHEB2 recombinant protein

Protein Construction: Recombinant Human RHEB is produced by our E Coli expression system and the target gene encoding Met1-Met184 is expressed with a GST tag at the N-terminus.

Background: GTP-Binding Protein Rheb (RHEB) is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. Highest levels of RHEB can be found in the skeletal and cardiac muscle, and it is vital in the regulation of growth and cell cycle progression due to its role in the Insulin/TOR/S6K signaling pathway. RHEB stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling, and it activates the protein kinase activity of mTORC1. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, farnesylation of the protein is required for this activity.

NCBI and Uniprot Product Information

• NCBI GI #: 6919957
• NCBI GeneID: 6009
• UniProt Accession #: Q15382
• Molecular Weight: Molecular Mass: 20.4kDa; Actual Protein Molecular Mass: 45kDa
• NCBI Official Full Name: GTP-binding protein Rheb
• NCBI Official Synonym Full Names: Ras homolog, mTORC1 binding
• NCBI Official Symbol: RHEB
• NCBI Official Synonym Symbols: RHEB2
• NCBI Protein Information: GTP-binding protein Rheb
• UniProt Protein Name: GTP-binding protein Rheb
• UniProt Gene Name: RHEB
• UniProt Synonym Gene Names: RHEB2
• UniProt Entry Name: RHEB_HUMAN

NCBI Description

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

RHEB: Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Ubiquitous. Highest levels observed in skeletal and cardiac muscle. Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2. Belongs to the small GTPase superfamily. Rheb family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rheb

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; spliceosome; endoplasmic reticulum membrane; membrane; cell soma; dendrite; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; metal ion binding; protein kinase binding

Biological Process: metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; signal transduction; cell cycle arrest; positive regulation of TOR signaling pathway