Isocitrate Dehydrogenase [NADP] Recombinant Protein | IDH1 recombinant protein

Recombinant Human Isocitrate Dehydrogenase [NADP] Cytoplasmic Protein

Cat. Num. AAA2556647

• Gene Names: IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Isocitrate Dehydrogenase [NADP]; Recombinant Human Isocitrate Dehydrogenase [NADP] Cytoplasmic Protein; Isocitrate Dehydrogenase [NADP] Cytoplasmic; IDH; Cytosolic NADP-Isocitrate Dehydrogenase; IDP; NADP(+)-Specific ICDH; Oxalosuccinate Decarboxylase; IDH1; PICD; IDH1 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 414

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Met1-Leu414
• Fusion Tag: C-6His
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt.; Please minimize freeze-thaw cycles.

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Related Product Information for IDH1 recombinant protein

Protein Construction: Recombinant Human Isocitrate Dehydrogenase [NADP] Cytoplasmic is produced by our Mammalian expression system and the target gene encoding Met1-Leu414 is expressed with a 6His tag at the C-terminus.

Background: Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer, binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid.

NCBI and Uniprot Product Information

• NCBI GI #: 538917488
• NCBI GeneID: 3417
• NCBI Accession #: NP_001269315.1
• NCBI GenBank Nucleotide #: NM_001282386.1
• UniProt Accession #: O75874; Q567U4; Q6FHQ6; Q7Z3V0; Q93090; Q9NTJ9; Q9UKW8
• Molecular Weight: Molecular Mass: 47.7kDa; Actual Protein Molecular Mass: 45kDa
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase (NADP(+)) 1, cytosolic
• NCBI Official Symbol: IDH1
• NCBI Official Synonym Symbols: IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• NCBI Protein Information: isocitrate dehydrogenase [NADP] cytoplasmic
• UniProt Protein Name: Isocitrate dehydrogenase [NADP] cytoplasmic
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH1
• UniProt Synonym Gene Names: PICD; IDH

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]