Interleukin-35 Recombinant Protein | EBI3 recombinant protein

Recombinant Human Interleukin-35 Protein

Cat. Num. AAA2552283

• Gene Names: EBI3; IL27B; IL35B; IL-27B
• Purity: >80% as determined by reducing SDS-PAGE.
• Synonyms: Interleukin-35; Recombinant Human Interleukin-35 Protein; Interleukin 35; IL35; hIL35; EBI3 recombinant protein

• Host: Human Cells
• Purity/Purification: >80% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Positions: Arg21-Lys229 & Arg23-Ser219
• Sequence Length: 229

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-Fc
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for EBI3 recombinant protein

The novel Ebi3-IL-12alpha heterodimeric cytokine has been designated interleukin-35 (IL-35), is a member IL12 family cytokine produced by regulatory T cells (Treg), but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of IL-12α (P35) and IL-27β chains, which are encoded by two separate genes called IL12A and EBI3 (Epstein-Barr-virus-induced gene 3) respectively. Ectopic expression of IL-35 confers regulatory activity on naive T cells, whereas recombinant IL-35 suppresses T-cell proliferation. It identify IL-35 as a novel inhibitory cytokine that may be specifically produced by T(reg) cells and is required for maximal suppressive activity. IL-35 has biological activity and able to expand CD4+CD25+ Treg cells, suppress the proliferation of CD4+CD25- effector cells and inhibit Th17 cell polarization. IL-35 has been shown to be constitutively expressed by regulatory T (Treg) cells CD4(+)CD25(+)Foxp3(+) and suggested to contribute to their suppressive activity. IL-35 is a crucial mediator which provokes CD4+CD25+ T cell proliferation and IL-10 generation, another well-known anti-inflammatory cytokine, along with TGFbeta cytokine. IL-35 is a cytokine can downregulate Th17 cell development and inhibit autoimmune inflammation. It inhibited the differentiation of Th17 cells in vitro. In vivo, IL-35 effectively attenuated established collagen-induced arthritis in mice, with concomitant suppression of IL-17 production but enhanced IFN-gamma synthesis. Thus, IL-35 is a novel anti-inflammatory cytokine suppressing the immune response through the expansion of regulatory T cells and suppression of Th17 cell development.

NCBI and Uniprot Product Information

• NCBI GI #: 14577917
• NCBI GeneID: 10148
• NCBI Accession #: NP_005746.2
• NCBI GenBank Nucleotide #: NM_005755.2
• UniProt Accession #: Q14213; O75269; A0N0N2
• Molecular Weight: Molecular Mass: 73.9 kDa; Actual Protein Molecular Mass: 90-120 kDa
• NCBI Official Full Name: interleukin-27 subunit beta
• NCBI Official Synonym Full Names: Epstein-Barr virus induced 3
• NCBI Official Symbol: EBI3
• NCBI Official Synonym Symbols: IL27B; IL35B; IL-27B
• NCBI Protein Information: interleukin-27 subunit beta
• UniProt Protein Name: Interleukin-27 subunit beta
• Protein Family: Interleukin
• UniProt Gene Name: EBI3
• UniProt Synonym Gene Names: IL27B; IL-27 subunit beta; IL-27B; EBV-induced gene 3 protein

NCBI Description

This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]

Uniprot Description

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.