Polo-like Kinase Antibody Kit | PLK1 antibody kit

Polo-like Kinase Antibody Sampler Kit

Cat. Num. AAA2550153

• Gene Names: PLK1; PLK; STPK13
• Applications: Western Blot
• Synonyms: Polo-like Kinase; Polo-like Kinase Antibody Sampler Kit; PLK1 antibody kit

• Sequence Length: 136

• Applicable Applications for PLK1 antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of NIH-3T3 cells with Plk1 Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of HepG2 cells with Fnk Polyclonal Antibody.)

Related Product Information for PLK1 antibody kit

Background: At least four distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4/SAK. PLK1 apparently plays many roles during mitosis, particularly in regulating mitotic entry and exit. The mitosis promoting factor (MPF), cdc2/cyclin B1, is activated by dephosphorylation of cdc2 (Thr14/Tyr15) by cdc25C. PLK1 phosphorylates cdc25C at Ser198 and cyclin B1 at Ser133 causing translocation of these proteins from the cytoplasm to the nucleus. PLK1 phosphorylation of Myt1 at Ser426 and Thr495 has been proposed to inactivate Myt1, one of the kinases known to phosphorylate cdc2 at Thr14/Tyr15. Polo-like kinases also phosphorylate the cohesin subunit SCC1, causing cohesin displacement from chromosome arms that allow for proper cohesin localization to centromeres. Mitotic exit requires activation of the anaphase promoting complex (APC), a ubiquitin ligase responsible for removal of cohesin at centromeres, and degradation of securin, cyclin A, cyclin B1, Aurora A, and cdc20. PLK1 phosphorylation of the APC subunits Apc1, cdc16, and cdc27 has been demonstrated in vitro and has been proposed as a mechanism by which mitotic exit is regulated. Substitution of Thr210 with Asp has been reported to elevate PLK1 kinase activity and delay/arrest cells in mitosis, while a Ser137Asp substitution leads to S-phase arrest. In addition, while DNA damage has been found to inhibit PLK1 kinase activity, the Thr210Asp mutant is resistant to this inhibition. PLK1 has been reported to be phosphorylated in vivo at Ser137 and Thr210 in mitosis; DNA damage prevents phosphorylation at these sites.

NCBI and Uniprot Product Information

• NCBI GI #: 1061144
• NCBI GeneID: 5347
• UniProt Accession #: P53350; Q15153; Q99746
• Molecular Weight: 68,255 Da
• NCBI Official Full Name: polo-like kinase, partial
• NCBI Official Synonym Full Names: polo like kinase 1
• NCBI Official Symbol: PLK1
• NCBI Official Synonym Symbols: PLK; STPK13
• NCBI Protein Information: serine/threonine-protein kinase PLK1
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PLK1
• UniProt Synonym Gene Names: PLK; PLK-1; STPK13

NCBI Description

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Uniprot Description

PLK1: a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events.

Protein type: EC 2.7.11.21; Kinase, protein; Other group; PLK family; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: centrosome; chromatin; condensed nuclear chromosome outer kinetochore; cytosol; kinetochore; microtubule cytoskeleton; midbody; nucleoplasm; nucleus; spindle; spindle microtubule; spindle midzone; spindle pole; synaptonemal complex

Molecular Function: anaphase-promoting complex binding; ATP binding; identical protein binding; kinase activity; magnesium ion binding; microtubule binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; centrosome cycle; establishment of protein localization; female meiosis chromosome segregation; G2/M transition of mitotic cell cycle; homologous chromosome segregation; microtubule bundle formation; mitotic cell cycle; mitotic cell cycle spindle assembly checkpoint; mitotic cytokinesis; mitotic nuclear envelope disassembly; mitotic sister chromatid segregation; negative regulation of apoptosis; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of transcription from RNA polymerase II promoter; nuclear envelope disassembly; peptidyl-serine phosphorylation; positive regulation of peptidyl-threonine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein localization to nucleus; positive regulation of proteolysis; positive regulation of ubiquitin protein ligase activity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein transferase activity; protein amino acid phosphorylation; protein destabilization; protein localization to chromatin; protein localization to nuclear envelope; protein ubiquitination; protein ubiquitination involved in ubiquitin-dependent protein catabolic process; regulation of cell cycle; regulation of cell cycle G2/M phase transition; regulation of cytokinesis; regulation of G2/M transition of mitotic cell cycle; regulation of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; regulation of mitotic spindle assembly; regulation of protein binding; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; signal transduction involved in G2 DNA damage checkpoint; sister chromatid cohesion