Western Blot (WB)
(Western Blot analysis of PC-3 cells using E-cadherin Monoclonal Antibody at dilution of 1:4000)
Mouse anti-Human, Mouse E-cadherin Monoclonal Antibody | anti-CDH1 antibody
E-cadherin Monoclonal Antibody
IHC: 1:50-1:500
IF: 1:50-1:500
Western Blot (WB)
(Western Blot analysis of PC-3 cells using E-cadherin Monoclonal Antibody at dilution of 1:4000)
Western Blot (WB)
(Western Blot analysis of PC-3 cells using E-cadherin Monoclonal Antibody at dilution of 1:4000)
NCBI and Uniprot Product Information
Observed Molecular Weight: 120kDa
NCBI Description
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Uniprot Description
CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.
Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: actin cytoskeleton; apical junction complex; catenin complex; cell junction; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasmic side of plasma membrane; endosome; extracellular exosome; extracellular region; flotillin complex; integral component of membrane; lamellipodium; lateral plasma membrane; perinuclear region of cytoplasm; plasma membrane; trans-Golgi network
Molecular Function: ankyrin binding; beta-catenin binding; cadherin binding; calcium ion binding; cell adhesion molecule binding; gamma-catenin binding; GTPase activating protein binding; identical protein binding; protein binding
Biological Process: cellular response to indole-3-methanol; cellular response to lithium ion; extracellular matrix disassembly; extracellular matrix organization; homophilic cell adhesion; negative regulation of cell-cell adhesion; neuron projection development; pituitary gland development; positive regulation of transcription factor import into nucleus; positive regulation of transcription, DNA-templated; protein localization to plasma membrane; response to drug; response to toxin; synaptogenesis
Disease: Blepharocheilodontic Syndrome 1; Breast Cancer; Endometrial Cancer; Gastric Cancer, Hereditary Diffuse; Ovarian Cancer; Prostate Cancer
Research Articles on CDH1
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Product Notes
The CDH1 cdh1 (Catalog #AAA2549068) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The E-cadherin Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's E-cadherin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:2000-1:20000 IHC: 1:50-1:500 IF: 1:50-1:500. Researchers should empirically determine the suitability of the CDH1 cdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E-cadherin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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