Rabbit Splicing factor 1 Polyclonal Antibody | anti-SF1 antibody
Phospho-Splicing factor 1 (Ser82) Polyclonal Antibody
IHC: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:5000
NCBI and Uniprot Product Information
Observed Molecular Weight: 68kDa
NCBI Description
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
Uniprot Description
SF1: a splicing factor necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. Phosphorylation of SF1 by cGMP-dependent protein kinase regulates spliceosome assembly. Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Six alternatively spliced isoforms have been described.
Protein type: RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: nuclear body; nucleoplasm; nucleus; ribosome; spliceosomal complex
Molecular Function: identical protein binding; pre-mRNA branch point binding; protein binding; RNA binding; transcription corepressor activity; zinc ion binding
Biological Process: Leydig cell differentiation; male sex determination; mRNA splicing, via spliceosome; negative regulation of smooth muscle cell proliferation; nuclear body organization and biogenesis; nuclear mRNA 3'-splice site recognition; regulation of steroid biosynthetic process; regulation of transcription, DNA-templated; spliceosomal complex assembly; transcription, DNA-dependent
Research Articles on SF1
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Product Notes
The SF1 sf1 (Catalog #AAA2548874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Splicing factor 1 (Ser82) Polyclonal Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Splicing factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:5000. Researchers should empirically determine the suitability of the SF1 sf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Splicing factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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