Rabbit Rb Polyclonal Antibody | anti-Rb antibody
Phospho-Rb (Thr826) Polyclonal Antibody
IHC: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:10000
NCBI and Uniprot Product Information
Observed Molecular Weight: 110kDa
NCBI Description
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
Uniprot Description
Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression.
Protein type: Nuclear receptor co-regulator; Oncoprotein; Transcription, coactivator/corepressor; Tumor suppressor
Chromosomal Location of Human Ortholog: 13q14.2
Cellular Component: chromatin; nucleoplasm; nucleus; PML body; Rb-E2F complex; spindle; SWI/SNF complex; transcription elongation factor complex b
Molecular Function: androgen receptor binding; DNA binding; DNA binding transcription factor activity; identical protein binding; kinase binding; phosphoprotein binding; protein binding; transcription coactivator activity; transcription factor binding; ubiquitin protein ligase binding
Biological Process: androgen receptor signaling pathway; cell cycle arrest; cell cycle checkpoint; cell division; cell morphogenesis involved in neuron differentiation; cellular response to xenobiotic stimulus; chromatin remodeling; covalent chromatin modification; enucleate erythrocyte differentiation; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; gut development; maintenance of mitotic sister chromatid cohesion; mitotic cell cycle checkpoint; myoblast differentiation; negative regulation of epithelial cell proliferation; negative regulation of G1/S transition of mitotic cell cycle; negative regulation of gene expression; negative regulation of protein kinase activity; negative regulation of smoothened signaling pathway; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter during mitotic cell cycle; negative regulation of transcription involved in G1/S transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; neuron apoptosis; neuron maturation; neuron projection development; positive regulation of macrophage differentiation; positive regulation of mitotic metaphase/anaphase transition; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription regulatory region DNA binding; positive regulation of transcription, DNA-templated; protein localization to chromosome, centromeric region; Ras protein signal transduction; regulation of cell growth; regulation of centromere complex assembly; regulation of cohesin loading; regulation of lipid kinase activity; regulation of mitotic cell cycle; regulation of transcription, DNA-templated; sister chromatid biorientation; striated muscle cell differentiation; tissue homeostasis; transcription, DNA-dependent; viral process
Disease: Bladder Cancer; Osteogenic Sarcoma; Retinoblastoma; Small Cell Cancer Of The Lung
Research Articles on Rb
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Product Notes
The Rb rb1 (Catalog #AAA2548866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Rb (Thr826) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rb can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the Rb rb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rb, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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