SDS-PAGE
TFPI / LACI / EPI recombinant protein
Recombinant Mouse TFPI / LACI / EPI Protein (His tag)
Gene Names
Tfpi; EPI; LACI; AW552122; A630013F22Rik
Purity
> 97 % as determined by SDS-PAGE
Synonyms
TFPI / LACI / EPI; Recombinant Mouse TFPI / LACI / EPI Protein (His tag); Tfpi; RP23-202K13.2; A630013F22Rik; AW552122; EPI; LACI; TFPI / LACI / EPI recombinant protein
Host
Human Cells
Purity/Purification
> 97 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
306
Application Notes
The secreted recombinant mouse TFPI consists of 272 amino acids and has a calculated molecular mass of 31 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45 kDa protein in SDS-PAGE under reducing conditions.
Predicted N Terminal
Leu 29
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for TFPI / LACI / EPI recombinant protein
Background: Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, Studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.
Description: A DNA sequence encoding the mouse TFPI isoform alpha (NP_035706.1) (Met 1-Lys 289) was expressed with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the mouse TFPI isoform alpha (NP_035706.1) (Met 1-Lys 289) was expressed with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,949 Da
NCBI Official Full Name
tissue factor pathway inhibitor isoform a
NCBI Official Synonym Full Names
tissue factor pathway inhibitor
NCBI Official Symbol
Tfpi
NCBI Official Synonym Symbols
EPI; LACI; AW552122; A630013F22Rik
NCBI Protein Information
tissue factor pathway inhibitor
UniProt Protein Name
Tissue factor pathway inhibitor
UniProt Gene Name
Tfpi
UniProt Synonym Gene Names
TFPI; EPI; LACI
Uniprot Description
TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Membrane protein, GPI anchor; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2|2 D
Cellular Component: cell surface; extracellular space
Research Articles on TFPI / LACI / EPI
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Product Notes
The TFPI / LACI / EPI tfpi (Catalog #AAA2547143) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant mouse TFPI consists of 272 amino acids and has a calculated molecular mass of 31 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45 kDa protein in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the TFPI / LACI / EPI tfpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFPI / LACI / EPI, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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