TLR3 / CD283 recombinant protein

Recombinant Mouse TLR3 / CD283 Protein (His tag)

Cat. Num. AAA2547132

• Gene Names: Tlr3; AI957183
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: TLR3 / CD283; Recombinant Mouse TLR3 / CD283 Protein (His tag); AI957183; Tlr3; TLR3 / CD283 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 905

• Application Notes: The secreted recombinant mouse TLR3 consists of 691 amino acids and has a calculated molecular mass of 78.7 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Thr 26
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for TLR3 / CD283 recombinant protein

Background: Toll-like receptor 3 (TLR3) also known as CD283 (cluster of differentiation 283) is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. TLR3/CD283 plays a fundamental role in pathogen recognition and activation of innate immunity. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses.

Description: A DNA sequence encoding the extracellular domain of mouse TLR3 (NP_569054.2) (Met 1-Leu 705) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 31543872
• NCBI GeneID: 142980
• NCBI Accession #: NP_569054.2
• NCBI GenBank Nucleotide #: NM_126166.4
• UniProt Accession #: Q99MB1; Q91ZM4
• Molecular Weight: 103,671 Da
• NCBI Official Full Name: toll-like receptor 3
• NCBI Official Synonym Full Names: toll-like receptor 3
• NCBI Official Symbol: Tlr3
• NCBI Official Synonym Symbols: AI957183
• NCBI Protein Information: toll-like receptor 3
• UniProt Protein Name: Toll-like receptor 3
• UniProt Gene Name: Tlr3

Uniprot Description

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 8|8 B1.1

Cellular Component: cell surface; cytoplasm; integral to plasma membrane; intracellular; lysosomal membrane

Molecular Function: double-stranded RNA binding; protein binding; receptor activity

Biological Process: activation of NF-kappaB transcription factor; defense response; defense response to virus; I-kappaB phosphorylation; innate immune response; microglial cell activation; microglial cell activation during immune response; positive regulation of chemokine biosynthetic process; positive regulation of chemokine production; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interferon-alpha biosynthetic process; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-beta production; positive regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of protein amino acid phosphorylation; positive regulation of toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; regulation of dendritic cell cytokine production; response to dsRNA; response to exogenous dsRNA; response to virus; toll-like receptor signaling pathway