HABP1 / C1QBP recombinant protein

Recombinant Mouse HABP1 / C1QBP Protein (His tag)

Cat. Num. AAA2547106

• Gene Names: C1qbp; P32; HABP1; gC1qBP; AA407365; AA986492; D11Wsu182e
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: HABP1 / C1QBP; Recombinant Mouse HABP1 / C1QBP Protein (His tag); SDPR; HABP1 / C1QBP recombinant protein

• Host: E Coli
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 279

• Application Notes: The recombinant mouse C1QBP consisting of 215 amino acids and has a calculated molecular mass of 24.7 kDa. rmC1QBP migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for HABP1 / C1QBP recombinant protein

Background: Hyaluronan binding protein 1 (HABP1), also known as p32 or gC1qR, is a ubiquitously expressed multifunctional phospho-protein implicated in cell signalling. Hyaluronan-binding protein 1 (HABP1) /p32/gC1qR was characterized as a highly acidic and oligomeric protein, which binds to different ligands like hyaluronan, C1q, and mannosylated albumin. The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro. HABP1 overexpressing cells showed extensive vacuolation and reduced growth rate, which was corrected by frequent medium replenishment. Further investigation revealed that HABP1 overexpressing cells undergo apoptosis, and they failed to enter into the S-phase. The sperm surface HABP1 level can be correlated with the degree of sperm motility.Hyaluronan binding protein 1 (HABP1) was reported to be present on human sperm surface and its involvement in fertilization has already been elucidated: decreased HABP1 level may be associated with low motility of sperms, which in turn might cause infertility in the patient. HABP1 also is an endogenous substrate for MAP kinase and upon mitogenic stimulation it is translocated to the nucleus in a MAP kinase-dependent manner.

Description: A DNA sequence encoding the mature form of mouse C1QBP (NP_031599.2) (Leu 72-Gln 279) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 112181167
• NCBI GeneID: 12261
• NCBI Accession #: NP_031599.2
• NCBI GenBank Nucleotide #: NM_007573.2
• UniProt Accession #: O35658
• Molecular Weight: 31,013 Da
• NCBI Official Full Name: complement component 1 Q subcomponent-binding protein, mitochondrial
• NCBI Official Synonym Full Names: complement component 1, q subcomponent binding protein
• NCBI Official Symbol: C1qbp
• NCBI Official Synonym Symbols: P32; HABP1; gC1qBP; AA407365; AA986492; D11Wsu182e
• NCBI Protein Information: complement component 1 Q subcomponent-binding protein, mitochondrial
• UniProt Protein Name: Complement component 1 Q subcomponent-binding protein, mitochondrial
• UniProt Gene Name: C1qbp
• UniProt Synonym Gene Names: Gc1qbp; C1qBP

Uniprot Description

C1QBP: a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. Originally identified via its binding interactions with Splicing Factor (SF-2). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases, the tumor suppressor ARF, and multiple antigens of bacterial and viral origin. Overexpressed in a number of cancer cell types, and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis. Inhibits the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress. Binding to C1q inhibits C1. In complex with cytokeratin-1/KRT1 is a high affinty receptor for kininogen-1/HMWK. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. The cell surface form may require docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. Belongs to the MAM33 family.

Protein type: Mitochondrial; Motility/polarity/chemotaxis; Nucleolus; Receptor, misc.

Chromosomal Location of Human Ortholog: 11 B4|11 43.21 cM

Cellular Component: cell surface; cytoplasm; cytosol; extracellular space; membrane; mitochondrion; nucleus

Molecular Function: adrenergic receptor binding; complement component C1q binding; hyaluronic acid binding; kininogen binding; mRNA binding; protein kinase C binding; transcription corepressor activity; transcription factor binding

Biological Process: mature ribosome assembly; negative regulation of defense response to virus; negative regulation of interferon-gamma production; negative regulation of interleukin-12 production; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of transcription from RNA polymerase II promoter; phosphoinositide 3-kinase cascade; positive regulation of apoptosis; positive regulation of cell adhesion; positive regulation of protein kinase B signaling cascade; regulation of complement activation