CD69 / CLEC2C / AIM recombinant protein

Recombinant Mouse CD69 Protein (His tag, ECD)

Cat. Num. AAA2546815

• Gene Names: Cd69; AIM; VEA; AI452015; 5830438K24Rik
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CD69 / CLEC2C / AIM; Recombinant Mouse CD69 Protein (His tag; ECD); 5830438K24Rik; AI452015; AIM; VEA; CLEC2C; CD69 / CLEC2C / AIM recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween 80.
• Sequence: Asn62-Arg199

• Application Notes: The recombinant mouse Cd69 consists 149 amino acids and predicts a molecular mass of 17.3 kDa.
• Species: Mouse
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
• Tag: C-His
• Reconstitution: It is recommended that sterile water (80uL) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months. This product is provided as lyophilized powder which is shipped with blue ice.

SDS-PAGE

Related Product Information for CD69 / CLEC2C / AIM recombinant protein

Background: Early activation antigen CD69, also known as activation inducer molecule (AIM), is a single-pass type II membrane protein. Recently, cDNA clones encoding human and mouse CD69 were isolated and showed CD69 to be a member of the C-type lectin superfamily. It is one of the earliest cell surface antigens expressed by T cells following activation. Once expressed, CD69 acts as a costimulatory molecule for T cell activation and proliferation. In addition to mature T cells, CD69 is inducibly expressed by immature thymocytes, B cells, natural killer (NK) cells, monocytes, neutrophils and eosinophils, and is constitutively expressed by mature thymocytes and platelets. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. The structure, chromosomal localization, expression and function of CD69 suggest that it is likely a pleiotropic immune regulator, potentially important in the activation and differentiation of a wide variety of hematopoietic cells. This membrane molecule transiently expresses on activated lymphocytes, and its selective expression in inflammatory infiltrates suggests that it plays a role in the pathogenesis of inflammatory diseases. CD69 plays a crucial role in the pathogenesis of allergen-induced eosinophilic airway inflammation and hyperresponsiveness and that CD69 could be a possible therapeutic target for asthmatic patients.

Description: A DNA sequence encoding the mouse Cd69 (NP_001028294.1) (Asn62-Arg199) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 84370294
• NCBI GeneID: 12515
• NCBI Accession #: NP_001028294.1
• NCBI GenBank Nucleotide #: NM_001033122.3
• UniProt Accession #: P37217
• Molecular Weight: Mol_Mass: 17.3 kDa
• NCBI Official Full Name: early activation antigen CD69
• NCBI Official Synonym Full Names: CD69 antigen
• NCBI Official Symbol: Cd69
• NCBI Official Synonym Symbols: AIM; VEA; AI452015; 5830438K24Rik
• NCBI Protein Information: early activation antigen CD69
• UniProt Protein Name: Early activation antigen CD69
• UniProt Gene Name: Cd69

Uniprot Description

CD69: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6 F3|6 63.21 cM

Cellular Component: external side of plasma membrane

Molecular Function: calcium ion binding