PARP-1 / PARP recombinant protein

Recombinant Mouse PARP-1 / PARP Protein (His tag)

Cat. Num. AAA2546804

• Gene Names: Parp1; PARP; PPOL; ARTD1; Adprp; Adprt1; C80510; parp-1; sPARP-1; AI893648; 5830444G22Rik
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: PARP-1 / PARP; Recombinant Mouse PARP-1 / PARP Protein (His tag); 5830444G22Rik; AI893648; Adprp; Adprt1; C80510; PARP; PPOL; parp-1; sPARP-1; PARP-1 / PARP recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly, 0.1mM TCEP
• Sequence Length: 1014

• Application Notes: The recombinant mouse PARP1 consists of 1033 amino acids and has a calculated molecular mass of 115 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PARP-1 / PARP recombinant protein

Background: Poly (ADP-ribose) polymerase 1 (PRAP1), also known as NAD (+) ADP-ribosyltransferase 1 (ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly (ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly (ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly (ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

Description: A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 20806109
• NCBI GeneID: 11545
• NCBI Accession #: NP_031441.2
• NCBI GenBank Nucleotide #: NM_007415.2
• Molecular Weight: 55,237 Da
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly (ADP-ribose) polymerase family, member 1
• NCBI Official Symbol: Parp1
• NCBI Official Synonym Symbols: PARP; PPOL; ARTD1; Adprp; Adprt1; C80510; parp-1; sPARP-1; AI893648; 5830444G22Rik
• NCBI Protein Information: poly [ADP-ribose] polymerase 1
• UniProt Protein Name: Poly [ADP-ribose] polymerase 1
• UniProt Gene Name: Parp1
• UniProt Synonym Gene Names: Adprp; Adprt; Adprt1; PARP-1; ARTD1; ADPRT 1; msPARP

Uniprot Description

PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Protein type: DNA repair, damage; EC 2.4.2.30; Nuclear envelope; Nuclear receptor co-regulator; Nucleolus; Transferase

Chromosomal Location of Human Ortholog: 1 H4|1 84.44 cM

Cellular Component: cytoplasm; membrane; mitochondrion; nuclear chromosome, telomeric region; nuclear envelope; nucleolus; nucleoplasm; nucleus; protein complex; transcription factor complex

Molecular Function: chromatin binding; DNA ligase (ATP) activity; enzyme binding; estrogen receptor binding; histone deacetylase binding; NAD binding; NAD+ ADP-ribosyltransferase activity; protein binding; protein kinase binding; protein N-terminus binding; SMAD binding; transcription factor binding

Biological Process: base-excision repair; behavioral response to cocaine; cellular response to insulin stimulus; DNA damage response, detection of DNA damage; DNA ligation during DNA repair; DNA metabolic process; DNA repair; double-strand break repair; lagging strand elongation; mitochondrial DNA metabolic process; mitochondrial DNA repair; mitochondrion organization and biogenesis; positive regulation of estrogen receptor signaling pathway; positive regulation of mitochondrial depolarization; positive regulation of transcription from RNA polymerase II promoter; protein amino acid ADP-ribosylation; protein autoprocessing; regulation of growth rate; response to DNA damage stimulus; telomere maintenance; transforming growth factor beta receptor signaling pathway