CD157 / BST1 recombinant protein

Recombinant Human CD157 / BST1 Protein (His tag)

Cat. Num. AAA2546556

• Gene Names: BST1; CD157
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: CD157 / BST1; Recombinant Human CD157 / BST1 Protein (His tag); CD157; BST1; CD157 / BST1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 318

• Application Notes: The secreted recombinant human BST1 comprises 275 amino acids with a predicted molecular mass of 31.4 kDa. As a result of glycosylation, rhBST1 migrates as an approximately 38-43 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly 29
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD157 / BST1 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O2-) production in the human U937 myeloid line.

Description: A DNA sequence encoding the human BST1 (NP_004325.2) (Met 1-Lys 292) with a C-terminal polyhistidine tag was expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 168229159
• NCBI GeneID: 683
• NCBI Accession #: NP_004325.2
• NCBI GenBank Nucleotide #: NM_004334.2
• UniProt Accession #: Q10588; Q1XII0; Q5U0K0; Q96EN3; B2R6A2
• Molecular Weight: 29,326 Da
• NCBI Official Full Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
• NCBI Official Synonym Full Names: bone marrow stromal cell antigen 1
• NCBI Official Symbol: BST1
• NCBI Official Synonym Symbols: CD157
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
• UniProt Gene Name: BST1
• UniProt Synonym Gene Names: BST-1; cADPr hydrolase 2

NCBI Description

Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq, Jul 2008]

Uniprot Description

BST1: Synthesizes cyclic ADP-ribose, a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. Belongs to the ADP-ribosyl cyclase family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.2.2.6; Hydrolase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: extrinsic to membrane; integrin complex; plasma membrane; uropod

Molecular Function: NAD+ nucleosidase activity; phosphorus-oxygen lyase activity

Biological Process: humoral immune response; positive regulation of B cell proliferation; positive regulation of cell proliferation; regulation of actin cytoskeleton organization and biogenesis; regulation of calcium-mediated signaling; regulation of cell-matrix adhesion; regulation of cellular extravasation; regulation of inflammatory response; regulation of peptidyl-tyrosine phosphorylation; signal transduction