COMP recombinant protein

Recombinant Human COMP Protein (His tag)

Cat. Num. AAA2546456

• Gene Names: COMP; MED; EDM1; EPD1; TSP5; PSACH; THBS5
• Purity: 83.8% as determined by reducing SDS-PAGE.
• Synonyms: COMP; Recombinant Human COMP Protein (His tag); EDM1; EPD1; MED; MGC131819; MGC149768; PSACH; THBS5; COMP recombinant protein

• Host: HEK293 Cells
• Purity/Purification: 83.8% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as sterile PBS, pH 7.4
• Sequence: Met 1-Ala 757
• Sequence Length: 757

• Application Notes: The mature form of human COMP consists of 748 amino acids after removal of the signal peptide and predicts a molecular mass of 82.4 kDa. As a result of glycosylation, the apparent molecular mass of rhCOMP is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 21
• Expression Host: HEK293 Cells.
• Protein Construction: A DNA sequence encoding the human COMP (NP_000086.2) precusor (Met 1-Ala 757) was expressed with a C-terminal polyhistidine tag.
• AP_Mol_Mass: 120-130 kDa
• Tag: C-his
• Endotoxin: < 1.0 EU per µg as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70°C .Store it under sterile conditions at -20° to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Testing Data(TD)

Related Product Information for COMP recombinant protein

Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellularmatrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressedprimarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with highaffinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growthplate organization and function. It is suggested to play a role in cell growth and development, and recent studies haverevealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor ofapoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia(PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.

NCBI and Uniprot Product Information

• NCBI GI #: 40217843
• NCBI GeneID: 1311
• NCBI Accession #: NP_000086.2
• NCBI GenBank Nucleotide #: NM_000095.2
• UniProt Accession #: P49747; O14592; Q16388; Q16389; Q2NL86; Q8N4T2; B4DKJ3
• Molecular Weight: 82.4 kDa
• NCBI Official Full Name: cartilage oligomeric matrix protein
• NCBI Official Synonym Full Names: cartilage oligomeric matrix protein
• NCBI Official Symbol: COMP
• NCBI Official Synonym Symbols: MED; EDM1; EPD1; TSP5; PSACH; THBS5
• NCBI Protein Information: cartilage oligomeric matrix protein
• UniProt Protein Name: Cartilage oligomeric matrix protein
• Protein Family: Complexin
• UniProt Gene Name: COMP
• UniProt Synonym Gene Names: COMP; TSP5

NCBI Description

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2016]

Uniprot Description

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: calcium ion binding; collagen binding; extracellular matrix structural constituent; heparan sulfate proteoglycan binding; heparin binding; protease binding; protein binding

Biological Process: extracellular matrix organization and biogenesis; limb development; negative regulation of apoptosis; organ morphogenesis; skeletal development

Disease: Epiphyseal Dysplasia, Multiple, 1; Pseudoachondroplasia