Leptin Receptor / LEPR / CD295 Recombinant Protein | LEPR / CD295 recombinant protein

Recombinant Human Leptin Receptor / LEPR / CD295 Protein (His tag)

Cat. Num. AAA2546340

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Leptin Receptor / LEPR / CD295; Recombinant Human Leptin Receptor / LEPR / CD295 Protein (His tag); LEPR; CD295; OBR; LEPR / CD295 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1165

• Application Notes: The recombinant human LEPR consists of 829 amino acids and has a predicted molecular mass of 95 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LEPR is approximately 130-140 kDa due to glycosylation.
• Predicted N Terminal: Phe 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for LEPR / CD295 recombinant protein

Background: Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is a transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms; Ob-Ra - Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction.

Description: A DNA sequence encoding the extracellular domain (Met 1-Asp 839) of human leptin receptor (NP_002294.2) was expressed, fused with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 40254464
• NCBI GeneID: 3953
• NCBI Accession #: NP_002294.2
• NCBI GenBank Nucleotide #: NM_002303.5
• UniProt Accession #: P48357; Q13592; Q13593; Q13594; Q92919; Q92920; Q92921
• Molecular Weight: 96,042 Da
• NCBI Official Full Name: leptin receptor isoform 1
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; LEPRD
• NCBI Protein Information: leptin receptor
• UniProt Protein Name: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R

NCBI Description

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: plasma membrane; receptor complex

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: angiogenesis; cell surface receptor linked signal transduction; energy reserve metabolic process; glucose homeostasis; leptin-mediated signaling pathway; multicellular organismal development; negative regulation of autophagy; regulation of bone remodeling; sexual reproduction; T cell differentiation

Disease: Leptin Receptor Deficiency